Almeida Luciane Madureira, Floriano Juliana Ferreira, Ribeiro Thuanne Pires, Magno Lais Nogueira, da Mota Lígia Souza Lima Silveira, Peixoto Nei, Mrué Fátima, Melo-Reis Paulo, Lino Junior Ruy de Souza, Graeff Carlos Frederico de Oliveira, Gonçalves Pablo José
Universidade Estadual de Goiás, Rodovia GO 330, km 241 - Anel Viário, S/N, Ipameri, CEP 75.780-000, Brazil,
J Mater Sci Mater Med. 2014 Sep;25(9):2153-62. doi: 10.1007/s10856-014-5255-8. Epub 2014 Jun 29.
The latex obtained from Hancornia speciosa is used in folk medicine for treatment of several diseases, such as acne, warts, diabetes, gastritis and inflammation. In this work, we describe the biocompatibility assessment and angiogenic properties of H. speciosa latex and its potential application in medicine. The physical-chemical characterization was carried out following different methodologies (CHN elemental analyses; thermogravimetric analyses and Fourier transform infrared spectroscopy). The biocompatibility was evaluated through cytotoxicity and genotoxicity tests in fibroblast mouse cells and the angiogenic properties were evaluated using the chick chorioallantoic membrane (CAM) assay model. The physical-chemical results showed that the structure of Hancornia speciosa latex biomembrane is very similar to that of Hevea brasiliensis (commercially available product). Moreover, the cytotoxicity and genotoxicity assays showed that H. speciosa latex is biocompatible with life systems and can be a good biomaterial for medical applications. The CAM test showed the efficient ability of H. speciosa latex in neovascularization of tissues. The histological analysis was in accordance with the results obtained in the CAM assay. Our data indicate that the latex obtained from H. speciosa and eluted in water showed significant angiogenic activity without any cytotoxic or genotoxic effects on life systems. The same did not occur with H. speciosa latex stabilized with ammonia. Addition of ammonia does not have significant effects on the structure of biomembranes, but showed a smaller cell survival and a significant genotoxicity effect. This study contributes to the understanding of the potentialities of H. speciosa latex as a source of new phytomedicines.
从美丽汉科木中提取的乳胶在民间医学中用于治疗多种疾病，如痤疮、疣、糖尿病、胃炎和炎症。在本研究中，我们描述了美丽汉科木乳胶的生物相容性评估和血管生成特性及其在医学中的潜在应用。采用不同方法（CHN元素分析、热重分析和傅里叶变换红外光谱）进行了物理化学表征。通过对成纤维细胞小鼠细胞进行细胞毒性和遗传毒性测试来评估生物相容性，并使用鸡胚绒毛尿囊膜（CAM）试验模型评估血管生成特性。物理化学结果表明，美丽汉科木乳胶生物膜的结构与巴西橡胶树（市售产品）的结构非常相似。此外，细胞毒性和遗传毒性试验表明，美丽汉科木乳胶与生命系统具有生物相容性，可成为医学应用的良好生物材料。CAM试验表明，美丽汉科木乳胶在组织新血管形成方面具有高效能力。组织学分析与CAM试验结果一致。我们的数据表明，从美丽汉科木中提取并用水洗脱的乳胶显示出显著的血管生成活性，对生命系统没有任何细胞毒性或遗传毒性作用。用氨稳定的美丽汉科木乳胶则没有这种情况。添加氨对生物膜结构没有显著影响，但显示出较低的细胞存活率和显著的遗传毒性作用。本研究有助于了解美丽汉科木乳胶作为新型植物药来源的潜力。
