Komatsu Fumio, Kagawa Yasuo, Ishiguro Kiyomi, Kawabata Terue, Purvee Baatar, Otgon Jugder, Chimedregzen Ulziiburen
Kagawa Nutrition University, 3-9-21, Chiyoda, Sakado, Saitama, 350-0288, Japan.
Curr Aging Sci. 2009 Mar;2(1):28-42. doi: 10.2174/1874609810902010028.
Oxidative stress induces several diseases and early aging. Previously, we reported that Mongolians are exposed in high oxidative stress, which may cause their early aging. In this study, to know the reason of high oxidative stress, we measured hair metals. This investigation was performed in Murun city, in the northern area of this country, and 469 healthy subjects, ranging from 10 to 82 years of age, were randomly enrolled. Oxidative stress was evaluated by the levels of serum reactive oxygen metabolites (ROM), malondialdehyde-modified low-density lipoprotein (MDA-LDL) and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG). Antioxidant capacity (AOC) was estimated by the levels of biological antioxidant potential (BAP) and superoxide dismutase (SOD) activity. Scalp hair metals were measured using an inductively coupled plasma mass spectrometry method. Murun subjects showed high ROM levels of 394+/-75 Carr U (n=342), compared with Japanese healthy subjects (n=356, 326+/-51 Carr U, p<0.001). MDA-LDL and 8-OHdG levels also showed high levels. While, BAP levels of Murun subjects were 2263+/-203 micromol/L (n=210), Japanese subjects (n=356, 2087+/-215 micromol/L, p<0.001). SOD activities were also high, suggesting that the high oxidative may accelerate the state of AOC. Murun subjects demonstrated high accumulation of several metals in the hairs. In particular, Mn accumulation exhibited from 2 fold to 40 fold increases of Japanese standard. These findings are indicative that the high Mn accumulation may contribute to the high oxidative stress. The mechanism of its high accumulation was not explained by food materials or drinking water. We should further investigate another influence such as sandy wind. In order to suppress the high oxidative stress, elimination of the high Mn accumulation should be urgently studied.
氧化应激会引发多种疾病和早衰。此前，我们报道过蒙古人面临高氧化应激，这可能导致他们早衰。在本研究中，为了解高氧化应激的原因，我们测量了头发中的金属含量。这项调查在该国北部的穆伦市进行，随机招募了469名年龄在10至82岁之间的健康受试者。通过血清活性氧代谢产物（ROM）、丙二醛修饰的低密度脂蛋白（MDA-LDL）和尿8-羟基-2'-脱氧鸟苷（8-OHdG）水平评估氧化应激。通过生物抗氧化潜力（BAP）水平和超氧化物歧化酶（SOD）活性估计抗氧化能力（AOC）。使用电感耦合等离子体质谱法测量头皮头发中的金属含量。与日本健康受试者（n = 356，326±51 Carr U，p < 0.001）相比，穆伦受试者的ROM水平较高，为394±75 Carr U（n = 342）。MDA-LDL和8-OHdG水平也较高。而穆伦受试者的BAP水平为2263±203微摩尔/升（n = 210），日本受试者（n = 356，2087±215微摩尔/升，p < 0.001）。SOD活性也较高，表明高氧化可能加速AOC状态。穆伦受试者头发中几种金属的积累量较高。特别是，锰的积累量比日本标准增加了2倍至40倍。这些发现表明，高锰积累可能导致高氧化应激。其高积累的机制无法通过食物或饮用水来解释。我们应该进一步研究其他影响因素，如风沙。为了抑制高氧化应激，应迫切研究消除高锰积累的方法。
