Ferrante Margherita, Fiore Maria, Conti Gea Oliveri, Fiore Valerio, Grasso Alfina, Copat Chiara, Signorelli Salvatore Santo
Department of Medical, Surgical and Advanced Technologies 'G.F. Ingrassia', University of Catania, I-95123 Catania, Italy.
Department of Clinical and Experimental Medicine, University of Catania, I-95123 Catania, Italy.
Mol Med Rep. 2017 May;15(5):3438-3444. doi: 10.3892/mmr.2017.6394. Epub 2017 Mar 24.
Our study compared copper (Cu), zinc (Zn) and manganese (Mn), crucial for human normal physio-logy maintenance, and lead (Pb) levels as environmental pollutant, in subjects suffering of deep vein thrombosis of lower limbs (DVTs) vs. healthy subjects. Furthermore, we evaluated oxidative stress parameters, the thiobarbituric acid reactive substances (TBARS) as the sum of malondialdehyde or 1,1,3,3-tetraethoxypropane (MDA) and 4-hydroxynonenal 4-HNE) and cytosolic superoxide dismutase (Cu/Zn-SOD) concentrations in both groups. We recruited 24 DVT cases and 46 healthy subjects as controls. Questionnaire with socio‑demographic, habits and lifestyle were collected. Hair concentrations of Zn, Mn, Cu and Pb were measured by ICP-MS, plasma concentrations of MDA and 4-HNE were measured by HPLC and SOD plasma concentrations were detected by ELISA test. A quantitative and qualitative variables comparison between cases and controls group was made by Mann-Whitney U test and Pearson's Chi-square test, respectively. We found low concentrations of Zn, Mn and Cu vs. high Pb concentrations in DVTs subjects. TBARS were found higher in the cases group, conversely, SOD concentrations were found lower in cases with respect to controls. Furthermore, we found the diet of pathological subjects significantly deficient in vegetables. These results are indicative of a lower enzymatic activity in patients, related to low transition metal levels in the DVTs and high levels of Pb, coupled with an unbalanced diet.
我们的研究比较了对于维持人体正常生理功能至关重要的铜（Cu）、锌（Zn）和锰（Mn），以及作为环境污染物的铅（Pb）在下肢深静脉血栓形成（DVT）患者与健康受试者体内的水平。此外，我们评估了两组的氧化应激参数，即硫代巴比妥酸反应性物质（TBARS），其为丙二醛或1,1,3,3 - 四乙氧基丙烷（MDA）与4 - 羟基壬烯醛（4 - HNE）的总和，以及胞质超氧化物歧化酶（Cu/Zn - SOD）的浓度。我们招募了24例DVT患者和46名健康受试者作为对照。收集了有关社会人口统计学、习惯和生活方式的问卷。通过电感耦合等离子体质谱法（ICP - MS）测量头发中Zn、Mn、Cu和Pb的浓度，通过高效液相色谱法（HPLC）测量血浆中MDA和4 - HNE的浓度，通过酶联免疫吸附测定（ELISA）检测血浆中SOD的浓度。分别采用曼 - 惠特尼U检验和皮尔逊卡方检验对病例组和对照组之间的定量和定性变量进行比较。我们发现DVT患者体内Zn、Mn和Cu浓度较低，而Pb浓度较高。病例组的TBARS较高，相反，与对照组相比，病例组的SOD浓度较低。此外，我们发现患病受试者的饮食中蔬菜明显不足。这些结果表明患者体内酶活性较低，这与DVT患者体内过渡金属水平低、Pb水平高以及饮食不均衡有关。
