噬菌体 T4 基因在肠杆菌科中的转移，在单细胞水平上确定。
Transfer of a phage T4 gene into Enterobacteriaceae, determined at the single-cell level.
机构信息
Faculty of Pharmacy, Osaka Ohtani University, 3-11-1 Nishikiori-kita, Tondabayashi 584-8540, Japan.
出版信息
Appl Environ Microbiol. 2010 Feb;76(4):1274-7. doi: 10.1128/AEM.02219-09. Epub 2009 Dec 18.
Abstract
The transfer range of phage genes was investigated at the single-cell level by using an in situ DNA amplification technique. After absorption of phages, a phage T4 gene was maintained in the genomes of non-plaque-forming bacteria at frequencies of 10(-2) gene copies per cell. The gene transfer decreased the mutation frequencies in nonhost recipients.
摘要
通过使用原位 DNA 扩增技术，在单细胞水平上研究了噬菌体基因的转移范围。在吸收噬菌体后，噬菌体 T4 基因以每个细胞 10(-2)个基因拷贝的频率保持在非噬菌斑形成细菌的基因组中。基因转移降低了非宿主受体的突变频率。
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