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基于直接成像的低温下蜡样芽胞杆菌 ATCC 14579 群体异质性定量分析。

Direct-imaging-based quantification of Bacillus cereus ATCC 14579 population heterogeneity at a low incubation temperature.

机构信息

Wageningen University and Research Centre, Laboratory of Food Microbiology, Wageningen, The Netherlands.

出版信息

Appl Environ Microbiol. 2010 Feb;76(3):927-30. doi: 10.1128/AEM.01372-09. Epub 2009 Dec 18.

DOI:10.1128/AEM.01372-09
PMID:20023091
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2813029/
Abstract

Bacillus cereus ATCC 14579 was cultured in microcolonies on Anopore strips near its minimum growth temperature to directly image and quantify its population heterogeneity at an abusive refrigeration temperature. Eleven percent of the microcolonies failed to grow during low-temperature incubation, and this cold-induced population heterogeneity could be partly attributed to the loss of membrane integrity of individual cells.

摘要

蜡样芽胞杆菌 ATCC 14579 在其最低生长温度附近的 Anopore 条上进行微菌落培养，以直接对其在恶劣冷藏温度下的种群异质性进行成像和定量分析。在低温孵育过程中，有 11%的微菌落未能生长，这种低温诱导的种群异质性部分归因于个别细胞的膜完整性丧失。

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