利用适体结构转换简便且经济高效地检测石房蛤毒素
Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching.
作者信息
Alfaro Karol, Bustos Paulina, O Sullivan Ciara, Conejeros Pablo
机构信息
Centro de Investigación y Gestión de Recursos Naturales, Facultad de Ciencias, Universidad de Valparaíso, Gran Bretańa 1111, Valparaíso, Chile.
Nanobiotechnology and Bioanalysis Group, Department of Chemical Engineering, Universitat Rovira i Virgili, 43007 ES-Tarragona, Spain.
出版信息
Food Technol Biotechnol. 2015 Sep;53(3):337-341. doi: 10.17113/ftb.53.03.15.3911.
Abstract
A simple method to detect saxitoxin (STX), one of the main components of the paralytic shellfish poison from red tide, has been developed. By using a next generation dye for double-stranded DNA we were able to differentiate fluorescence from STX-binding aptamers when exposed to different concentrations of STX, suggesting a change in aptamer folding upon target binding. The developed method is extremely rapid, only requiring small sample volumes, with quantitative results in the concentration range of 15 ng/mL to 3 µg/mL of STX, with a detection limit of 7.5 ng/mL.
摘要
已开发出一种简单的方法来检测麻痹性贝类毒素（PST）的主要成分之一——石房蛤毒素（STX），该毒素源于赤潮。通过使用一种用于双链DNA的新一代染料，当暴露于不同浓度的STX时，我们能够区分STX结合适体的荧光，这表明靶标结合后适体折叠发生了变化。所开发的方法极其快速，只需要少量样品体积，在15 ng/mL至3 µg/mL的STX浓度范围内可获得定量结果，检测限为7.5 ng/mL。
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