Christian-Doppler Laboratory for Molecular Recognition Materials, Department of Analytical Chemistry and Food Chemistry, University of Vienna, Waehringer Strasse 38, 1090, Vienna, Austria.
Anal Bioanal Chem. 2010 May;397(1):147-160. doi: 10.1007/s00216-009-3340-5. Epub 2009 Dec 19.
In the present paper, we report on the development of a straightforward reversed-phase liquid chromatography-electrospray ionization-tandem mass spectrometry method for the determination of the most abundant fatty acids; alpha-tocopherol and cephalosporin P1 in fermentation broths. Using this method, fatty acids could be successfully determined in extracts of fermentation broths from penicillin and cephalosporin production without prior derivatization. Matrix effects were investigated in detail, and various kinds of calibrations (i.e., by use of neat standard solutions as well as by matrix-matched calibration employing standard addition each with and without internal standards) were comparatively assessed. The optimized and validated method was employed for the analysis of extracts of fermentation broths and nutrition media.
在本文中，我们报告了一种直接的反相液相色谱-电喷雾串联质谱法的开发，用于测定最丰富的脂肪酸；α-生育酚和头孢菌素 P1 在发酵液中。使用这种方法，可以在青霉素和头孢菌素生产发酵液的提取物中成功地测定脂肪酸，而无需事先衍生化。详细研究了基质效应，并比较了各种校准方法（即用纯标准溶液以及用基质匹配校准，分别使用和不使用内部标准的标准添加）。优化和验证后的方法用于发酵液和营养培养基提取物的分析。
