Analysis of the Impact of Ethanol Extract of L. on Human Fibroblast Cell Cultures Using the PANsys 3000 Device for Breeding and Visualization of Cells.

L. promotes wound healing and might be effective in gingival fibroblast stimulation. The influence of different concentrations of L. ethanol extract on human gingival fibroblast was visualized using PANsys 3000-a fully automated cell culture device used for in vitro culture to study cells under conditions similar to in vivo. The human fibroblast cells were isolated from gingival tissue. The 100% brew of L., as well as 7% and 20% L. ethanol extract, were added to the cultured cells and observed for 72 h. The qualitative and quantitative composition of the volatile compounds of marigold L. flowers are presented in this study. The essential oil compounds of the decoction were isolated with solid-phase microextraction (SPME) and analyzed with gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The presence of terpenoids, flavonoids, and other compounds was demonstrated. The composition was correlated with the fragrance properties. Observation of gingival fibroblast showed that there were no changes in cell morphology and proliferation after 100% L. brew stimulation. The growth and cell division were not inhibited. Likewise, the addition of 7% or 20% ethanol in water extract of L. stimulation did not inhibit the fibroblast proliferation. Overall, ethanol extracts of L. decrease the alcohol cytotoxic influence on gingival fibroblasts.