Eren Belgin Ezgi, Delibalta Cankız Gizem
Department of Chemistry, Faculty of Science, Muğla Sıtkı Koçman University, Muğla, Turkiye.
Biomaterials and Tissue Engineering Laboratory, Muğla Sıtkı Koçman University Research Laboratories Center, Muğla, Turkiye.
Turk J Chem. 2023 Oct 16;47(6):1529-1539. doi: 10.55730/1300-0527.3633. eCollection 2023.
Quaternized chitosan (HTCC) was synthesized and characterized to increase chitosan solubility. Then HTCC was electrospun with poly (vinyl alcohol) (PVA) and prepared natural bioactive agent () extract was loaded onto fibers for wound scaffold applications. Morphological, structural, and mechanical characterization of the produced wound scaffolds was performed and their in vitro bioactive component release behavior was investigated. As a result, it was observed that the degree of quaternization of chitosan was 0.89, and synthesized HTCC was soluble in acidic, basic, alkaline media and could be electrospun with PVA in the presence of a natural bioactive agent. The presence of HTCC increased Young's modulus and the tensile strength of the PVA scaffolds, while the presence of bioactive extract caused a decrease in Young's modulus and an increase in tensile strength. is released in a controlled and slow manner from the scaffolds within approximately 55 h. The release behavior was consistent with the Higuchi kinetic model. In this study, the effect of PVA cooperator on HTCC nanofiber production in the presence of a bioactive component was investigated for the first time. HTCC and extract were also used together for the first time in the composition of a fiber scaffold. The mechanical properties and release kinetics of these scaffolds were also investigated for the first time. According to the results, it is thought that the wound scaffolds produced have the potential to be used as a new treatment tool, especially for chronic wounds.
合成并表征了季铵化壳聚糖（HTCC）以提高壳聚糖的溶解度。然后将HTCC与聚乙烯醇（PVA）进行静电纺丝，并将制备的天然生物活性剂提取物负载到纤维上用于伤口支架应用。对所制备的伤口支架进行了形态、结构和力学表征，并研究了它们在体外生物活性成分的释放行为。结果表明，壳聚糖的季铵化程度为0.89，合成的HTCC可溶于酸性、碱性、碱性介质中，并且在天然生物活性剂存在下可与PVA进行静电纺丝。HTCC的存在提高了PVA支架的杨氏模量和拉伸强度，而生物活性提取物的存在则导致杨氏模量降低和拉伸强度增加。在大约55小时内，从支架中以可控且缓慢的方式释放。释放行为符合Higuchi动力学模型。在本研究中，首次研究了PVA助剂在生物活性成分存在下对HTCC纳米纤维生产的影响。HTCC和提取物也首次一起用于纤维支架的组成中。还首次研究了这些支架的力学性能和释放动力学。根据结果，认为所制备的伤口支架有潜力用作一种新的治疗工具，特别是用于慢性伤口。
