Department of Biochemistry, Faculty of Medicine and Medical Sciences, Arabian Gulf University (AGU), PO Box 26671, Manama, Kingdom of Bahrain.
Malar J. 2009 Dec 22;8:306. doi: 10.1186/1475-2875-8-306.
Plasmodium falciparum malaria is a complex disease in which genetic and environmental factors influence susceptibility. IgG isotypes are in part genetically controlled, and GM/KM allotypes are believed to be involved in this control.
In this study, 216 individuals from Daraweesh, an area of seasonal malaria transmission in Sudan, were followed for nine years for malaria infection. Total IgG and IgG isotypes against four malaria antigens, MSP2-3D7, MSP2-FC27, AMA1, and Pf332-C231 were measured in plasma obtained from the cohort at the end of the study, during the dry malaria-free period. The GM/KM allotypes of the donors were determined.
The GM 1,17 5,13,14,6 phenotype was associated with a higher incidence of malaria compared with the non-1,17 5,13,14,6 phenotypes (P = 0.037). Paradoxically, the carriers of the GM 1,17 5,13,14,6 phenotype had significantly higher baseline levels of total IgG and non-cytophilic IgG isotypes as compared to non-carriers. The KM allotypes influence on IgG isotypes level was limited. Finally, the differences in the baseline concentrations of total IgG and IgG isotypes between the different GK/KM phenotype carriers were antigen-dependent.
The results show that GM but not KM allotypes appeared to influence host susceptibility to uncomplicated malaria as well as the antibody profile of the donors, and the carriers of the GM 1,17 5,13,14,6 phenotype were the most susceptible
The GM allotypes have significant influence on susceptibility to uncomplicated P. falciparum malaria and antigen-dependent influence on total IgG and IgG subclasses.
恶性疟原虫疟疾是一种复杂的疾病，其中遗传和环境因素影响易感性。IgG 同种型部分受遗传控制，GM/KM 同种异型被认为参与这种控制。
在这项研究中，对来自苏丹季节性疟疾传播地区 Daraweesh 的 216 名个体进行了为期 9 年的疟疾感染随访。在研究结束时，即无疟疾的旱季，从该队列中采集血浆，测量针对四种疟疾抗原（MSP2-3D7、MSP2-FC27、AMA1 和 Pf332-C231）的总 IgG 和 IgG 同种型。测定供体的 GM/KM 同种异型。
与非 1,17 5,13,14,6 表型相比，GM 1,17 5,13,14,6 表型与疟疾发病率较高相关（P=0.037）。矛盾的是，GM 1,17 5,13,14,6 表型携带者的总 IgG 和非细胞毒性 IgG 同种型的基线水平明显高于非携带者。KM 同种异型对 IgG 同种型水平的影响有限。最后，不同 GK/KM 表型携带者之间总 IgG 和 IgG 同种型的基线浓度差异取决于抗原。
结果表明，GM 而不是 KM 同种异型似乎影响宿主对无并发症疟疾的易感性以及供体的抗体谱，GM 1,17 5,13,14,6 表型携带者最易感染。
GM 同种异型对无并发症恶性疟原虫疟疾的易感性有显著影响，并对总 IgG 和 IgG 亚类有抗原依赖性影响。
