Lee Tin-Lap, Cheung Hoi-Hung, Claus Janek, Sastry Chandan, Singh Sumeeta, Vu Loc, Rennert Owen, Chan Wai-Yee
Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Nucleic Acids Res. 2009 Jan;37(Database issue):D891-7. doi: 10.1093/nar/gkn644. Epub 2008 Oct 2.
GermSAGE is a comprehensive web-based database generated by Serial Analysis of Gene Expression (SAGE) representing major stages in mouse male germ cell development, with 150,000 sequence tags in each SAGE library. A total of 452,095 tags derived from type A spermatogonia (Spga), pachytene spermatocytes (Spcy) and round spermatids (Sptd) were included. GermSAGE provides web-based tools for browsing, comparing and searching male germ cell transcriptome data at different stages with customizable searching parameters. The data can be visualized in a tabulated format or further analyzed by aligning with various annotations available in the UCSC genome browser. This flexible platform will be useful for gaining better understanding of the genetic networks that regulate spermatogonial cell renewal and differentiation, and will allow novel gene discovery. GermSAGE is freely available at http://germsage.nichd.nih.gov/
GermSAGE是一个基于网络的综合数据库，由基因表达序列分析（SAGE）生成，代表小鼠雄性生殖细胞发育的主要阶段，每个SAGE文库中有150,000个序列标签。总共纳入了来自A型精原细胞（Spga）、粗线期精母细胞（Spcy）和圆形精子细胞（Sptd）的452,095个标签。GermSAGE提供基于网络的工具，用于浏览、比较和搜索不同阶段的雄性生殖细胞转录组数据，搜索参数可定制。数据可以以表格形式可视化，或通过与UCSC基因组浏览器中可用的各种注释进行比对进一步分析。这个灵活的平台将有助于更好地理解调节精原细胞更新和分化的遗传网络，并将有助于发现新基因。可通过http://germsage.nichd.nih.gov/免费访问GermSAGE。
