Clinic for Acupuncture, Erlangen, Germany.
J Neurosci Res. 2010 Jun;88(8):1645-50. doi: 10.1002/jnr.22329.
Acupuncture can alleviate symptoms of spinal cord injuries (SCI). The underlying mechanism, however, is unknown. We hypothesized that stem cells could be mobilized by acupuncture. Therefore, we enrolled 14 healthy study participants using acupuncture points for the treatment of SCI. The frequency of CD133 and CD34 cells in peripheral blood and the serum concentrations of matrix metalloproteinase (MMP)-9, brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and interleukin-6 were determined before and after acupuncture (<1 hr, 24 hr, and 48 hr). CD133(+)34(-) cells were doubled 48 hr after acupuncture, with concomitant decreases in BDNF and MMP-9 levels. Interleukin-6 remained below detectable levels, eliminating a stress-induced cell release. Individuals acupunctured on control counterpoints showed no changes in CD133(+) cells. Our results indicate that acupuncture for SCI can mobilize human CD133(+)34(-) cells. (c) 2009 Wiley-Liss, Inc.
针灸可以缓解脊髓损伤（SCI）的症状。然而，其潜在机制尚不清楚。我们假设针灸可以动员干细胞。因此，我们招募了 14 名健康的研究参与者，使用针灸穴位治疗 SCI。在针灸前（<1 小时、24 小时和 48 小时）和针灸后测定外周血中 CD133 和 CD34 细胞的频率和基质金属蛋白酶（MMP）-9、脑源性神经营养因子（BDNF）、神经生长因子（NGF）和白细胞介素-6 的血清浓度。针灸后 48 小时，CD133(+)34(-)细胞增加了一倍，同时 BDNF 和 MMP-9 水平降低。白细胞介素-6 仍处于检测不到的水平，排除了应激诱导的细胞释放。在对照穴位进行针灸的个体中，CD133(+)细胞没有变化。我们的结果表明，针灸治疗 SCI 可以动员人类 CD133(+)34(-)细胞。（c）2009 年 Wiley-Liss, Inc.
