Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Spine (Phila Pa 1976). 2012 Jun 1;37(13):E768-77. doi: 10.1097/BRS.0b013e318246d59c.
Experimental animal study of spinal cord injury (SCI), using a cell delivery system.
To investigate the therapeutic effects of transplantation of peripheral blood-derived CD133 cells, with a magnetic delivery system in a rat SCI model.
There are no reports on intrathecal transplantation of peripheral blood-derived CD133 cells, with a magnetic cell delivery system to treat SCI.
Magnetically isolated peripheral blood-derived CD133 cells were used as the cell source. Contusion SCI was induced by an Infinite Horizon impactor in athymic nude rats. CD133 cells or phosphate-buffered saline was administered via a lumbar puncture immediately after SCI, and a magnetic field was applied to rats for 30 minutes. Animals were analyzed at specific times after transplantation by several methods to examine cell tracking, functional recovery, and histological angiogenesis and neurogenesis.
A combination of cell transplantation and application of a magnetic field at the site of injury caused significant functional recovery. Transplantation of the cells alone in the absence of the magnetic field showed no effect beyond that observed in control rats.
The combination of intrathecal transplantation of CD133 cells and application of a magnetic field at the site of injury is a possible therapeutic strategy to treat rat SCI and may therefore find application in clinical settings.
脊髓损伤（SCI）的实验动物研究，使用细胞递送系统。
探讨磁递送系统中移植外周血源性 CD133 细胞对大鼠 SCI 模型的治疗效果。
尚无鞘内移植外周血源性 CD133 细胞，用磁细胞递送系统治疗 SCI 的报道。
将磁分离的外周血源性 CD133 细胞用作细胞来源。在无胸腺裸鼠中通过 Infinite Horizon 撞击器诱导挫伤性 SCI。SCI 后立即通过腰椎穿刺给予 CD133 细胞或磷酸盐缓冲盐水，并对大鼠施加磁场 30 分钟。在移植后的特定时间，通过多种方法分析动物，以检查细胞追踪、功能恢复以及组织学血管生成和神经发生。
细胞移植与损伤部位磁场应用的结合导致显著的功能恢复。单独进行细胞移植而不应用磁场，除了在对照组大鼠中观察到的效果外，没有任何作用。
鞘内移植 CD133 细胞与损伤部位磁场应用的结合是治疗大鼠 SCI 的一种可能的治疗策略，因此可能在临床环境中得到应用。
