Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China ; Department of Neurosurgery, Second Hospital of Tianjin Medical University, Tianjin 300070, China.
Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China.
Neural Regen Res. 2012 Feb 5;7(4):278-82. doi: 10.3969/j.issn.1673-5374.2012.04.007.
The prominin-1/CD133 epitope is expressed in undifferentiated cells. Studies have reported that craniocerebral trauma in animal models of fluid percussion injury induces production of a specific stem cell subgroup. It has been hypothesized that fluid percussion injury induces CD133(+) cell infiltration in the brain tissue. The present study established a traumatic brain injury model through fluid percussion injury. Immunohistochemical staining showed significantly increased CD133 antigen expression in the rat brain following injury. CD133(+) cells were mainly distributed in hippocampal CA1-3 regions, as well as the dentate gyrus and hilus, of the lesioned hemisphere. Occasional cells were also detected in the cortex. In addition, reverse transcription-PCR revealed that no change in CD133 mRNA expression in injured brain tissue. These results suggested that fluid percussion injury induced CD133 antigen expression in the brain tissues as a result of conformational epitope changes, but not transcriptional expression.
Prominin-1/CD133 表位在未分化细胞中表达。研究报道，在流体冲击伤动物模型中颅脑创伤会诱导产生特定的干细胞亚群。有人假设，流体冲击伤会诱导 CD133(+)细胞浸润脑组织。本研究通过流体冲击伤建立了创伤性脑损伤模型。免疫组化染色显示，损伤后大鼠脑组织中 CD133 抗原表达显著增加。CD133(+)细胞主要分布于损伤半球的海马 CA1-3 区、齿状回和门区，偶尔也可见于皮质区。此外，逆转录-PCR 显示，损伤脑组织中 CD133 mRNA 表达无变化。这些结果表明，流体冲击伤诱导脑组织中 CD133 抗原表达是由于构象表位变化，而不是转录表达。
