He Xue-li, Liu Ti, Zhao Li-li
College of Life Sciences, Hebei University, Baoding 071002, Hebei, China.
Ying Yong Sheng Tai Xue Bao. 2009 Sep;20(9):2118-22.
A pot culture with unsterilized soil as growth substrate showed that AM fungi had significant effects on the growth of Astragalus membranaceus (Fabaceae) under different N application levels. Inoculation with AM fungi promoted the AM infection of A. membranaceus roots, but high N application level suppressed the infection. Inoculating AM fungi increased the growth rate, soluble protein and sugar contents, and SOD, POD and CAT activities of A. membranaceus. Under 50 and 100 mg x kg(-1) of N application, new bands of POD isozyme occurred in inoculated plants, and the contents of flavonoid, N, and P in A. membranaceus also had definite increase. The best inoculation effect was observed under the N application level of 50-100 mg N x kg(-1) soil.
以未灭菌土壤为生长基质的盆栽试验表明,在不同施氮水平下,丛枝菌根(AM)真菌对膜荚黄芪(豆科)的生长有显著影响。接种AM真菌促进了膜荚黄芪根系的AM侵染,但高施氮水平抑制了这种侵染。接种AM真菌提高了膜荚黄芪的生长速率、可溶性蛋白和糖含量,以及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性。在施氮量为50和100 mg x kg(-1)时,接种植株中出现了新的POD同工酶条带,膜荚黄芪中黄酮类化合物、氮和磷的含量也有一定增加。在土壤施氮量为50 - 100 mg N x kg(-1)时观察到最佳接种效果。