Université Bordeaux 1, UMR 5805 CNRS, Station Marine d'Arcachon, Place du Dr. Peyneau, Arcachon 33120, France.
Mar Pollut Bull. 2010 Apr;60(4):515-25. doi: 10.1016/j.marpolbul.2009.11.013. Epub 2009 Dec 22.
The present study evaluated the interactive effects of cadmium contamination and pathogenic organisms (trematodes Himasthla elongata and bacteria Vibrio tapetis) singularly and in combination during 7 days on the bivalve Cerastoderma edule. Some defense-related activities were analyzed such as genetic expression, metallothionein and immune responses. Trematode metacercarial infection, similar whatever the treatment, induced the strongest responses of immune parameters. Particularly, the interaction between cadmium and parasite exposures induced unusual responses on gene expression and immune responses. No effect of bacterial challenge appeared on bivalve responses, nevertheless a strong mortality of V. tapetis infected cockles occurred between 7 and 14 days. Cadmium bioaccumulation was significantly modulated by both pathogenic organisms. Furthermore, an antagonistic effect of trematodes and bacteria was shown on metal bioaccumulation of co-infected cockles. These results highlighted the importance of considering the multiplicity of perturbation sources in coastal ecosystems to assess the health status of organisms.
本研究评估了在 7 天内，单一及联合的镉污染和致病生物（吸虫中华分支睾吸虫和细菌创伤弧菌）对双壳类贻贝 Cerastoderma edule 的相互作用影响。分析了一些与防御相关的活动，如基因表达、金属硫蛋白和免疫反应。类似无论处理方式如何，吸虫尾蚴感染都会引起最强的免疫参数反应。特别是，镉和寄生虫暴露的相互作用诱导了基因表达和免疫反应的异常反应。细菌的挑战对双壳类动物的反应没有影响，但在 7 至 14 天之间，感染创伤弧菌的贻贝死亡率很高。两种致病生物均显著调节了镉的生物积累。此外，还显示了吸虫和细菌对共感染贻贝金属生物积累的拮抗作用。这些结果强调了在沿海生态系统中考虑多种干扰源来评估生物体健康状况的重要性。
