Fertility Centers of Illinois, Chicago, IL 60610, USA.
Hum Reprod. 2010 Mar;25(3):690-6. doi: 10.1093/humrep/dep442. Epub 2009 Dec 22.
Various experiments suggest that ovarian follicular recruitment and growth may be increased by testosterone priming. Our aim was to determine the effects of exogenous testosterone supplementation in older women on ovarian folliculogenesis and steroidogenesis.
A prospective randomized double-blind placebo-controlled crossover study was carried out. Twelve regularly menstruating non-obese women aged 38-45 years received a 12-day course of transdermal testosterone (2.5 mg per patch) or placebo patch, followed by 7 days of gonadotrophin stimulation. After at least a 1 month washout period, subjects underwent the same protocol using the opposite treatment. The main outcomes were follicular development (ultrasound measures) and hormone levels.
Following gonadotrophin stimulation, there were no differences in average number of follicles over 10 mm diameter in cycles pre-treated with testosterone versus placebo [2.10 (95% confidence interval (CI) 1.11, 3.22) versus 2.08 (95% CI 1.03, 3.14), P = 0.55]. No crossover, period (first or second test) or sequence (order of treatment) effects were noted. As expected, total and free testosterone levels were increased following testosterone treatment (312.7 +/- 122.4 versus 12.3 +/- 4.5 ng/dl and 45.5+/- 16.7 versus 1.4 +/- 0.5 ng/dl, respectively, P < 0.001) but no differences in free or total testosterone were noted by period. LH, FSH, estradiol and antral follicle counts before gonadotrophin stimulation were not altered by testosterone pretreatment or by period.
Despite increased testosterone levels, a short course of androgens had no significant effect on the number of follicles over 10 mm during stimulation with FSH in women of late reproductive age.
多项实验表明，睾酮预处理可能会增加卵巢卵泡募集和生长。我们的目的是确定外源性睾酮补充对老年女性卵巢卵泡发生和类固醇生成的影响。
进行了一项前瞻性随机双盲安慰剂对照交叉研究。12 名月经规律、非肥胖的 38-45 岁女性接受了为期 12 天的经皮睾酮（每贴 2.5 毫克）或安慰剂贴剂治疗，随后进行 7 天的促性腺激素刺激。至少经过 1 个月的洗脱期后，受试者使用相反的治疗方法进行相同的方案。主要结局是卵泡发育（超声测量）和激素水平。
在促性腺激素刺激后，经睾酮预处理与安慰剂预处理的周期中，直径大于 10mm 的卵泡平均数无差异[2.10（95%置信区间 1.11-3.22）与 2.08（95%置信区间 1.03-3.14），P=0.55]。未观察到交叉、周期（第一次或第二次测试）或顺序（治疗顺序）效应。正如预期的那样，睾酮治疗后总睾酮和游离睾酮水平升高[312.7+/-122.4 与 12.3+/-4.5ng/dl 和 45.5+/-16.7 与 1.4+/-0.5ng/dl，分别为 P<0.001]，但周期内游离或总睾酮无差异。促性腺激素刺激前的 LH、FSH、雌二醇和窦卵泡计数不受睾酮预处理或周期的影响。
尽管睾酮水平升高，但在晚期生育年龄女性中，短期应用雄激素对 FSH 刺激时直径大于 10mm 的卵泡数量没有显著影响。
