Bawm Saw, Tiwananthagorn Saruda, Lin Kyaw San, Hirota Junichi, Irie Takao, Htun Lat Lat, Maw Ni Ni, Myaing Tin Tin, Phay Nyunt, Miyazaki Satoshi, Sakurai Tatsuya, Oku Yuzaburo, Matsuura Hideyuki, Katakura Ken
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University.
J Vet Med Sci. 2010 Apr;72(4):525-8. doi: 10.1292/jvms.09-0508. Epub 2009 Dec 22.
Current chemotherapeutic options for African trypanosomiasis in humans and livestock are very limited. In the present study, a total of 71 medicinal plant specimens from 60 plant species collected in Myanmar were screened for antitrypanosomal activity against trypomastigotes of Trypanosoma evansi and cytotoxicity against MRC-5 cells in vitro. The methanol extract of dried rootbark of Vitis repens showed the highest antitrypanosomal activity with IC(50) value of 8.6 +/- 1.5 microg/ml and the highest selectivity index of 24.4. The extracts of Brucea javanica, Vitex arborea, Eucalyptus globulus and Jatropha podagrica had also remarkable activity with IC(50) values and selectivity indices in the range of 27.2-52.6 microg/ml and 11.4-15.1 respectively.
目前用于治疗人类和家畜非洲锥虫病的化疗方法非常有限。在本研究中，对从缅甸采集的60种植物的71个药用植物标本进行了体外筛选，检测其对伊氏锥虫无鞭毛体的抗锥虫活性以及对MRC-5细胞的细胞毒性。白背葡萄干燥根皮的甲醇提取物显示出最高的抗锥虫活性，IC(50)值为8.6±1.5微克/毫升，最高选择性指数为24.4。鸦胆子、钝叶黄荆、蓝桉和珊瑚花的提取物也具有显著活性，IC(50)值和选择性指数分别在27.2-52.6微克/毫升和11.4-15.1范围内。
