Department of Orthopedics, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, People's Republic of China.
Calcif Tissue Int. 2010 Feb;86(2):163-71. doi: 10.1007/s00223-009-9328-5. Epub 2009 Dec 23.
An experiment was designed to investigate whether systemic administration of tetracyclines (TCs) as bone fluorochrome labels could interfere with bone modeling in vivo and inhibit osteoclast formation and activity in vitro. Cell cultures of rat bone marrow macrophages revealed that TC and oxytetracycline inhibited osteoclastogenesis and bone resorption and stimulated apoptosis. Forty rats in five groups were treated with saline, calcein green, alizarin red S, TC, or oxytetracycline. Their tibias were used for histomorphometric analysis, including bone static, dynamic, and resorption parameters in the tibial proximal metaphysis. No significant differences in bone volume per tissue volume, trabecular number, trabecular thickness, trabecular separation, bone formation rate per bone surface, mineralizing surface, or mineral apposition rate were observed. TC or oxytetracycline decreased eroded surface, number of osteoclasts per bone perimeter, and osteoclast surface per bone surface by about 50%. The results demonstrated that TC and oxytetracycline inhibit rat osteoclast formation and activity in vitro, and histomorphometric parameters involved in bone turnover may be affected by the use of oxytetracycline and TC as fluorescent bone labels in vivo.
一项实验旨在研究全身给予四环素（TCs）作为骨荧光标记物是否会干扰体内骨形成，并抑制体外破骨细胞的形成和活性。大鼠骨髓巨噬细胞的细胞培养显示 TC 和土霉素抑制破骨细胞生成和骨吸收，并刺激细胞凋亡。将 40 只大鼠分为 5 组，分别给予生理盐水、钙黄绿素绿、茜素红 S、TC 或土霉素。使用胫骨近端干骺端的组织形态计量学分析包括骨静态、动态和吸收参数，对其胫骨进行处理。组织学参数中，骨体积/组织体积、骨小梁数量、骨小梁厚度、骨小梁分离、骨表面形成率、矿化表面和矿化沉积率均无显著差异。TC 或土霉素使每个骨周缘的破骨细胞数和每个骨表面的破骨细胞表面积减少约 50%。结果表明，TC 和土霉素抑制大鼠体外破骨细胞的形成和活性，并且骨转换涉及的组织形态计量学参数可能会受到体内使用土霉素和 TC 作为荧光骨标记物的影响。
