Cheng Wenxiang, Yue Ye, Fan Wenbin, Hu Yiping, Wang Xinluan, Pan Xiaohua, Zhou Xiaoxiao, Qin Ling, Zhang Peng
Translational Medicine R&D Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Pharmazie. 2012 May;67(5):457-9.
Tetracyclines have been widely used in bone histomorphometry to label new bone formation and apposition rate. However, most studies of tetracyclines have also shown their strong inhibitory action on osteoclasts and their effects on osteoblast activities as well. To even obtain the in-depth understanding on this issue, we have reviewed related studies in "Pubmed" by searching the keywords "tetracyclines and osteoclast", "tetracyclines and osteoblast", which retrieved 118 and 162 related documents, respectively. Among these papers, some described the application of tetracyclines as fluorescent marker in bone histomorphometry, while others discussed their role in protection of bone metabolism partly through inhibiting osteoclastogenesis or bone resorption and through enhancing osteogenesis. Based on the above mentioned, it seems that tetracyclines used as bone labeling markers may affect the results of bone histomorphometry to some extent. To even confirm the effect of tetracyclines on bones cells (osteoblast, osteoclast) and in vivo bone remodeling, related research work has been performed in our research team which indicated quite different results in vivo and in vitro. Therefore, the influence of tetracyclines on bones may differ in terms of different conditions which need to be further elucidated.
四环素已广泛应用于骨组织形态计量学，以标记新骨形成和骨沉积速率。然而，大多数关于四环素的研究也表明其对破骨细胞有强烈的抑制作用，并且对成骨细胞活性也有影响。为了更深入地了解这个问题，我们通过在“Pubmed”中搜索关键词“四环素与破骨细胞”、“四环素与成骨细胞”，回顾了相关研究，分别检索到118篇和162篇相关文献。在这些论文中，一些描述了四环素作为荧光标记物在骨组织形态计量学中的应用，而另一些则讨论了它们在保护骨代谢方面的作用，部分是通过抑制破骨细胞生成或骨吸收以及通过增强成骨作用。基于上述情况，似乎用作骨标记物的四环素可能在一定程度上影响骨组织形态计量学的结果。为了进一步证实四环素对骨细胞（成骨细胞、破骨细胞）和体内骨重塑的影响，我们研究团队开展了相关研究工作，结果表明体内和体外的结果差异很大。因此，四环素对骨的影响可能因不同条件而异，这需要进一步阐明。
