Department of Physiology and Pharmacology, University of Bristol, UK.
Cerebellum. 2010 Jun;9(2):218-31. doi: 10.1007/s12311-009-0152-2.
In the present study, a non-pulsatile arterially perfused hindbrain and upper body rat preparation is described which is an extension of the brainstem preparation reported by Potts et al., (Brain Res Bull 53(1):59-67), 1. The modified in situ preparation allows study of cerebellar function whilst preserving the integrity of many of its interconnections with the brainstem, upper spinal cord and the peripheral nervous system of the head and forelimbs. Evoked mossy fibre, climbing fibre and parallel fibre field potentials and EMG activity elicited in forelimb biceps muscle by interpositus stimulation provided evidence that both cerebellar inputs and outputs remain operational in this preparation. Similarly, the spontaneous and evoked single unit activity of Purkinje cells, putative Golgi cells, molecular interneurones and cerebellar nuclear neurones was similar to activity patterns reported in vivo. The advantages of the preparation include the ability to record, without the complications of anaesthesia, stabile single unit activity for extended periods (3 h or more), from regions of the rat cerebellum that are difficult to access in vivo. The preparation should therefore be a useful adjunct to in vitro and in vivo studies of neural circuits underlying cerebellar contributions to movement control and motor learning.
在本研究中，描述了一种非脉动性动脉灌流的后脑和上身大鼠制备方法，该方法是由 Potts 等人报道的脑干制备方法的扩展。（Brain Res Bull 53(1):59-67）。经过改良的原位制备方法允许在保留其与脑干、上脊髓和头部及前肢外周神经系统的许多连接完整性的情况下研究小脑功能。由间位核刺激引起的诱发苔藓纤维、攀附纤维和平行纤维场电位以及前肢二头肌肌肉的肌电图活动提供了证据，表明在该制备中，小脑的输入和输出仍然是可行的。同样，浦肯野细胞、假定的高尔基细胞、分子中间神经元和小脑核神经元的自发和诱发的单个单位活动与体内报道的活动模式相似。该制备的优点包括能够在没有麻醉并发症的情况下记录稳定的单个单位活动，时间可延长（3 小时或更长时间），并且可以从活体中难以进入的大鼠小脑区域进行记录。因此，该制备方法应该是研究小脑对运动控制和运动学习贡献的神经回路的体外和体内研究的有用辅助手段。
