Chlewicki Lukasz K, Kumar Vinay
Department of Pathology, The University of Chicago, Chicago, IL, USA.
Methods Mol Biol. 2010;612:177-98. doi: 10.1007/978-1-60761-362-6_12.
Study of NK cell receptor signaling in mouse NK cells has been difficult since there are no clones of murine NK cells. We describe here a model system that overcomes this problem. This system allows the study of many aspects of NK cell receptor function with complete control over the variables that may affect activity such as cis versus trans ligand engagement, homotypic interactions, multiple target types, receptor number, receptor-ligand affinity, and signaling adaptor molecule expression. Although we give examples only for 2B4, Ly49C, and CD48, any NK cell receptors could be studied using these methods. Since many NK cell receptors such as 2B4, CD48, and the Ly49 family can be expressed in T cells, this model system allows the study of not only NK cells but also T cells with NK cell receptors. A standardized system for determining the regulation of NK cell receptor signaling can be important for understanding the anti-tumor activities of NK cells.
由于没有小鼠自然杀伤（NK）细胞的克隆，对小鼠NK细胞中NK细胞受体信号传导的研究一直很困难。我们在此描述了一个克服这一问题的模型系统。该系统能够在完全控制可能影响活性的变量（如顺式与反式配体结合、同型相互作用、多种靶标类型、受体数量、受体 - 配体亲和力以及信号衔接分子表达）的情况下，研究NK细胞受体功能的多个方面。尽管我们仅给出了2B4、Ly49C和CD48的示例，但使用这些方法可以研究任何NK细胞受体。由于许多NK细胞受体（如2B4、CD48和Ly49家族）可以在T细胞中表达，因此该模型系统不仅可以研究NK细胞，还可以研究表达NK细胞受体的T细胞。一个用于确定NK细胞受体信号传导调节的标准化系统对于理解NK细胞的抗肿瘤活性可能很重要。
