The effect of phytohormones on safflower regeneration plant.

OBJECTIVE

To study the effect of phytohormones on the regeneration of safflower, in order to provide a foundation for the rapid propagation of safflower seeds and genetic transformation.

METHODS

The experiment used the cotyledons as the explant and studied the influence of calli induction, differentiation and rooting by phytohormones.

RESULTS

1. Different hormones by single using induced calli, but didn't directly produce adventitious buds; 2. With the combination of different phytohormones on the differentiation of adventitious buds were significantly different, the roots were also significantly different; 3. Cotyledon calli induction medium: MS + 3% Sucrose + 0.7% Agar + 1.0 mg/L NAA; differentiation medium: MS + 3% Sucrose + 0.7% Agar + 0.2 mg/L NAA + 1.0 mg/L 6-BA; rooting medium: 1/4 MS + 2.0 mg/L NAA + 0.5 mg/L IAA.

CONCLUSION

We have screened out the best culture medium of calli induction, differentiation and rooting via using singularity phytohormone and two phytohormones.