Lu Ai-Fang, Qi Ming-Jun, Li Zong-Liang, Lv Hong-Fei
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
Zhong Yao Cai. 2010 Jul;33(7):1042-5.
The feasibility of producting flavonoids from callus of Tetrastigma hemsleyanum was investigated through callus induction, proliferation, differentiation and determination of flavonoids.
The leaves of sterile plantlet, leaves and stems of wild plants were used as explants to induce calluses; The root tuber, the leaves and calluses were selected for the determination of flavonoids. With ethanol as the solvent, the total flavonoids were extracted by ultrasonic and determined by spectrophotometry at 500 nm after stained with NaNO2-Al(NO3) 3.
The optimum medium where the calluses were induced was 2/3MS +2.0 mg/L 6-BA +2.0 mg/L NAA; MS +2.0 mg/L 6-BA +2.0 mg/L NAA was the optimum for callus proliferation; for callus root differentiation, the optimum medium was 1/2MS +1.0 mg/L 6-BA +1.0 mg/L NAA. The content of total flavonoids was 31.121 mg/g in root tuber, 12.830 mg/g in leaves while it was up to 18.088 mg/g in calluses.
The calluses had a high level of total flavonoids, it could produce flavonoids through the calluses induced by Tetrastigma hemsleyanum in a large scale. In that case, the pressing requirement in medical market will be solved.
通过三叶青愈伤组织的诱导、增殖、分化及黄酮类化合物的测定，研究从三叶青愈伤组织中生产黄酮类化合物的可行性。
以无菌苗叶片、野生植株叶片和茎段为外植体诱导愈伤组织；选取块根、叶片和愈伤组织进行黄酮类化合物的测定。以乙醇为溶剂，采用超声法提取总黄酮，经亚硝酸钠-硝酸铝染色后于500nm处用分光光度法测定。
诱导愈伤组织的最佳培养基为2/3MS +2.0mg/L 6-BA +2.0mg/L NAA；MS +2.0mg/L 6-BA +2.0mg/L NAA是愈伤组织增殖的最佳培养基；对于愈伤组织生根分化，最佳培养基为1/2MS +1.0mg/L 6-BA +1.0mg/L NAA。块根中总黄酮含量为31.121mg/g，叶片中为12.830mg/g，而愈伤组织中高达18.088mg/g。
愈伤组织中总黄酮含量较高，可通过三叶青诱导的愈伤组织大规模生产黄酮类化合物。如此，可解决医药市场的迫切需求。
