Bae Hyunsu, Kim Hye-Jeoung, Shin Minkyu, Lee Hyejung, Yin Chang Shik, Ra Jehyeon, Kim Jinju
Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Korea.
Neurol Res. 2010 Feb;32 Suppl 1:53-7. doi: 10.1179/016164109X12537002794002.
Agrimoniae Herba has been used as an anti-inflammatory agent in traditional medicine. Nitric oxide and proinflammatory cytokines produced by activated microglia may be a possible etiological factor of neurodegenerative disorders. We evaluated whether Agrimoniae Herba could have an anti-inflammatory effect on lipopolysaccharide-induced BV2 microglial cells.
The effects of Agrimoniae Herba on lipopolysaccharide-induced nitric oxide and proinflammatory cytokine production in BV2 microglial cells were evaluated by nitric oxide assay, enzyme-linked immunosorbent assay and western blotting.
Agrimoniae Herba had no cytotoxicity and suppressed lipopolysaccharide-induced nitric oxide production in BV2 microglial cells. Agrimoniae Herba also suppressed lipopolysaccharide-induced production of proinflammatory cytokines such as tumor necrosis factor, interleukin 1 beta and interleukin 6 in a dose-dependent manner. Agrimoniae Herba inhibited the expression of inducible nitric oxide synthase.
Taken together, these findings indicate that Agrimoniae Herba may be used as a form of pharmaceutical acupuncture therapy in the treatment of brain inflammation.
仙鹤草在传统医学中一直被用作抗炎剂。活化小胶质细胞产生的一氧化氮和促炎细胞因子可能是神经退行性疾病的一个潜在病因。我们评估了仙鹤草是否对脂多糖诱导的BV2小胶质细胞具有抗炎作用。
通过一氧化氮测定、酶联免疫吸附测定和蛋白质印迹法评估仙鹤草对脂多糖诱导的BV2小胶质细胞中一氧化氮和促炎细胞因子产生的影响。
仙鹤草无细胞毒性，并抑制脂多糖诱导的BV2小胶质细胞中一氧化氮的产生。仙鹤草还以剂量依赖的方式抑制脂多糖诱导的促炎细胞因子如肿瘤坏死因子、白细胞介素1β和白细胞介素6的产生。仙鹤草抑制诱导型一氧化氮合酶的表达。
综上所述，这些发现表明仙鹤草可能作为药物针刺疗法的一种形式用于治疗脑部炎症。
