Lee Gi-Ja, Yin Chang Shik, Choi Seok-Keun, Choi Samjin, Yang Jong-Soo, Lee Hyejung, Park Hun-Kuk
Department of Biomedical Engineering and Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemum-gu, Seoul 130-701, Republic of Korea.
Neurol Res. 2010 Feb;32 Suppl 1:79-83. doi: 10.1179/016164109X12537002794200.
Acupuncture has been known to be effective in ischemia, and glutamate excitotoxicity is an important factor for the neuronal cell death. We examined the effect of acupuncture on glutamate level in the ischemic stroke model.
A global ischemia was induced using the method of 11-vessel occlusion in rat. Rats were randomly divided into two groups: the control group (n=5, with 11-vessel occlusion) and the acupuncture group (n=5, with 11-vessel occlusion + acupuncture). The extracellular glutamate level was assessed using an intracerebral biosensor system measuring 256 samples per second. Simultaneously, the cerebral blood flow was measured and the electroencephalogram data were recorded. The time schedule of the experiment was as follows: 10 minutes of baseline measurement, 10 minutes of occlusion and 30 minutes of follow-up monitoring. Acupuncture stimulation was applied to the acupuncture points GB34 (Yangneungcheon) and GB39 (Hyeonjong) during the occlusion period.
In the control group, the extracellular glutamate level was changed as 135.19 +/- 23.76 microM (mean +/- standard deviation) from the baseline level. However, this increase was suppressed in the acupuncture group (72.20 +/- 27.15 microM, p<0.01 versus the control group). The changes of cerebral blood flow and electroencephalogram were not significantly different between the groups.
This result suggests that the effect of acupuncture might be closely associated with modulation of the brain glutamate release in the ischemic condition.
已知针刺在缺血方面有效，而谷氨酸兴奋性毒性是神经元细胞死亡的一个重要因素。我们研究了针刺对缺血性中风模型中谷氨酸水平的影响。
采用大鼠11根血管闭塞法诱导全脑缺血。大鼠随机分为两组：对照组（n = 5，行11根血管闭塞）和针刺组（n = 5，行11根血管闭塞 + 针刺）。使用每秒测量256个样本的脑内生物传感器系统评估细胞外谷氨酸水平。同时，测量脑血流量并记录脑电图数据。实验时间安排如下：基线测量10分钟，闭塞10分钟，随访监测30分钟。在闭塞期间对穴位GB34（阳陵泉）和GB39（悬钟）进行针刺刺激。
对照组中，细胞外谷氨酸水平相对于基线水平变化为135.19±23.76微摩尔（平均值±标准差）。然而，针刺组中这种升高受到抑制（72.20±27.15微摩尔，与对照组相比p<0.01）。两组之间脑血流量和脑电图的变化无显著差异。
该结果表明针刺的作用可能与缺血状态下脑谷氨酸释放的调节密切相关。
