Wang Wei, Ma Ting, Lian Kehui, Zhang Yue, Tian Huimei, Ji Kaihua, Li Guoqiang
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, P. R. China ; TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, P. R. China ; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, P. R. China.
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, P. R. China.
PLoS One. 2013 Dec 19;8(12):e84386. doi: 10.1371/journal.pone.0084386. eCollection 2013.
Sulfur can be removed from benzothiophene (BT) by some bacteria without breaking carbon-carbon bonds. However, a clear mechanism for BT desulfurization and its genetic components have not been reported in literatures so far. In this study, we used comparative transcriptomics to study differential expression of genes in Gordonia terrae C-6 cultured with BT or sodium sulfate as the sole source of sulfur. We found that 135 genes were up-regulated with BT relative to sodium sulfate as the sole sulfur source. Many of these genes encode flavin-dependent monooxygenases, alkane sulfonate monooxygenases and desulfinase, which perform similar functions to those involved in the 4S pathway of dibenzothiophene (DBT) biodesulfurization. Three of the genes were found to be located in the same operon, designated bdsABC. Cell extracts of pET28a-bdsABC transfected E. coli Rosetta (DE3) converted BT to a phenolic compound, identified as o-hydroxystyrene. These results advance our understanding of enzymes involved in the BT biodesulfurization pathway.
某些细菌能够在不破坏碳-碳键的情况下从苯并噻吩（BT）中去除硫。然而，迄今为止，文献中尚未报道BT脱硫的明确机制及其遗传成分。在本研究中，我们使用比较转录组学来研究以BT或硫酸钠作为唯一硫源培养的戈登氏菌C-6中基因的差异表达。我们发现，相对于以硫酸钠作为唯一硫源，有135个基因在以BT为硫源时上调表达。这些基因中的许多编码黄素依赖性单加氧酶、烷磺酸盐单加氧酶和脱亚磺酰酶，它们的功能与二苯并噻吩（DBT）生物脱硫4S途径中涉及的酶类似。其中三个基因位于同一个操纵子中，命名为bdsABC。转染了pET28a-bdsABC的大肠杆菌Rosetta (DE3)的细胞提取物将BT转化为一种酚类化合物，鉴定为邻羟基苯乙烯。这些结果增进了我们对BT生物脱硫途径中所涉及酶的理解。
