University of Naples Federico II, Department of Physics, via Cinthia, Napoli, Italy.
Methods. 2010 May;51(1):27-36. doi: 10.1016/j.ymeth.2009.12.009. Epub 2009 Dec 24.
This work reports on the application of Raman spectroscopy for the analysis of Xenopus laevis oocytes (stage-I). A two-color home-made microscope has been used for this investigation. In particular, a 785nm Raman probe has been used to acquire the spontaneous Raman scattering from the oocyte cytoplasm, while a 532nm probe has been employed to detect carotenoids through Resonant Raman Scattering. Finally, the distribution of beta-carotene along a diameter of a single oocyte has been investigated.
本工作报道了拉曼光谱在分析非洲爪蟾卵母细胞（I 期）中的应用。为此研究使用了一台自制的双色显微镜。具体来说，使用 785nm 的拉曼探头获取卵母细胞质体的自发拉曼散射，而使用 532nm 的探头通过共振拉曼散射来检测类胡萝卜素。最后，研究了单个卵母细胞直径上β-胡萝卜素的分布。
