Department of Biology, College of Life Science and Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-Gu, Seoul 120-749, Republic of Korea.
Semin Cell Dev Biol. 2010 Apr;21(2):156-62. doi: 10.1016/j.semcdb.2009.12.009. Epub 2009 Dec 24.
Under defined laboratory conditions, Naegleria gruberi undergo an amoeba-to-flagellate differentiation. During this differentiation, N. gruberi changes its shape from an amorphous amoeba to a regular shaped flagellate and forms de novo a flagellar apparatus, which is composed of two basal bodies, two flagella, a flagellar rootlet, and cytoplasmic microtubules. The entire process is accomplished within 2h after initiation of differentiation and more than 95% of cells in the population undergo this differentiation. This rapid and synchronous differentiation of N. gruberi provides us with a unique system in which we can study the process of de novo basal body assembly. In this review, I summarize recent findings associated with de novo basal body assembly and propose a hypothesis to explain how N. gruberi assemble two basal bodies per cell, which is what happens in the majority of cells.
在明确的实验室条件下，棘阿米巴原虫会经历从变形虫到鞭毛体的分化。在这个分化过程中，棘阿米巴原虫从无定形的变形虫转变为规则形状的鞭毛体，并重新形成鞭毛器，由两个基体、两条鞭毛、鞭毛根和细胞质微管组成。整个过程在分化开始后 2 小时内完成，种群中超过 95%的细胞都会经历这种分化。棘阿米巴原虫的这种快速和同步的分化为我们提供了一个独特的系统，我们可以用它来研究从头组装基体的过程。在这篇综述中，我总结了与从头组装基体相关的最新发现，并提出了一个假设来解释棘阿米巴原虫如何在每个细胞中组装两个基体，这也是大多数细胞中发生的情况。
