Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
J Cell Sci. 2010 Dec 1;123(Pt 23):4024-31. doi: 10.1242/jcs.077453. Epub 2010 Nov 2.
Naegleria gruberi is a single-celled eukaryote best known for its remarkable ability to form an entire microtubule cytoskeleton de novo during its metamorphosis from an amoeba into a flagellate, including basal bodies (equivalent to centrioles), flagella and a cytoplasmic microtubule array. Our publicly available full-genome transcriptional analysis, performed at 20-minute intervals throughout Naegleria differentiation, reveals vast transcriptional changes, including the differential expression of genes involved in metabolism, signaling and the stress response. Cluster analysis of the transcriptional profiles of predicted cytoskeletal genes reveals a set of 55 genes enriched in centriole components (induced early) and a set of 82 genes enriched in flagella proteins (induced late). The early set includes genes encoding nearly every known conserved centriole component, as well as eight previously uncharacterized, highly conserved genes. The human orthologs of at least five genes localize to the centrosomes of human cells, one of which (here named Friggin) localizes specifically to mother centrioles.
内格里虫是一种单细胞真核生物，以其在从变形虫到鞭毛虫的变态过程中从头形成完整的微管细胞骨架的非凡能力而闻名，包括基体（相当于中心粒）、鞭毛和细胞质微管阵列。我们公开提供的全基因组转录分析，在整个内格里虫分化过程中每隔 20 分钟进行一次，揭示了巨大的转录变化，包括参与代谢、信号转导和应激反应的基因的差异表达。预测细胞骨架基因的转录谱聚类分析揭示了一组 55 个基因富集在中心粒成分（早期诱导），一组 82 个基因富集在鞭毛蛋白（晚期诱导）。早期组包括编码几乎所有已知保守中心粒成分的基因，以及八个以前未表征的高度保守基因。至少五个基因的人类同源物定位于人类细胞的中心体，其中一个（在此命名为 Friggin）特异性定位于母中心粒。
