Section of Otolaryngology, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA.
Am J Otolaryngol. 2011 Jan-Feb;32(1):32-7. doi: 10.1016/j.amjoto.2009.09.010. Epub 2009 Dec 24.
Biofilms play a role in the pathogenesis of a variety of otorhinolaryngologic diseases, including otitis media and cholesteatoma. Despite this, relatively few studies have undertaken to demonstrate the presence of biofilms tissues from patients with chronic otitis media or infected cholesteatoma.
OBJECTIVE/HYPOTHESIS: Our objective is to detect evidence of biofilms human chronic ear infections with scanning electron microscopy (SEM). We hypothesized that bacterial biofilms are present in patients with chronic otitis media.
We performed prospective collection of tissue collected during middle ear surgery from 16 patients undergoing middle ear or mastoid surgery with chronic ear infections.
A total of 31 middle and mastoid tissue samples were harvested at the time of surgery and processed with critical point drying for SEM analysis. Samples were then searched for evidence of biofilms.
Bacterial-shaped objects were identified that displayed both surface binding and the presence of a glycocalyx in 4 patients, findings consistent with bacterial biofilms. Most of these (3 of 4) were in patients with infected cholesteatoma, and biofims were identified in 60% of cholesteatoma cases (3 of 5). On the other hand, only 1 of 7 cases with chronic suppurative otitis media had evidence of biofilms.
SEM supports the hypothesis that bacterial biofilms are common in chronic infections associated with cholesteatoma and are present in some cases of chronic suppurative otitis media without cholesteatoma.
生物膜在多种耳鼻喉疾病的发病机制中起作用，包括中耳炎和胆脂瘤。尽管如此，相对较少的研究致力于证明生物膜在慢性中耳炎或感染性胆脂瘤患者的组织中存在。
目的/假设：我们的目的是使用扫描电子显微镜（SEM）检测慢性耳部感染中生物膜的证据。我们假设慢性中耳炎患者存在细菌生物膜。
我们前瞻性地收集了 16 名接受中耳或乳突手术的慢性耳部感染患者在中耳手术期间收集的组织。
在手术时共采集了 31 个中耳和乳突组织样本，并进行临界点干燥处理，以进行 SEM 分析。然后，对样本进行生物膜存在的证据进行搜索。
在 4 名患者中发现了具有表面结合和糖萼存在的细菌形状物体，这与细菌生物膜一致。这些患者中有 3 名（4 名中的 3 名）患有感染性胆脂瘤，胆脂瘤病例中有 60%（5 名中的 3 名）存在生物膜。另一方面，慢性化脓性中耳炎的 7 例中仅有 1 例有生物膜证据。
SEM 支持以下假设，即细菌生物膜在与胆脂瘤相关的慢性感染中很常见，并且在一些无胆脂瘤的慢性化脓性中耳炎病例中存在。
