Unit of Ophthalmology, School of Clinical Sciences, University of Liverpool and St Paul's Eye Unit, Royal Liverpool University Hospital, Liverpool L69 3GA, UK.
Exp Eye Res. 2010 Mar;90(3):461-4. doi: 10.1016/j.exer.2009.12.007. Epub 2009 Dec 27.
This study sought to determine the distribution of opticin, an extracellular matrix small leucine-rich repeat protein secreted by the non-pigmented ciliary body epithelium (CBE), in pathological eye tissues including posterior hyaloid membranes (PHM) and epiretinal membranes (ERM) from subjects with proliferative diabetic retinopathy (PDR), central retinal vein occlusion (CRVO) and proliferative vitreoretinopathy (PVR). Eight enucleated eyes and eleven surgically excised PHMs/ERMs from patients with PDR, CRVO or PVR were analysed by immunohistochemistry for the presence and distribution of opticin, vitreous (delineated by a type II collagen antibody) and blood vessels (using CD31 and CD34 antibodies as endothelial markers). Opticin was present at the basal surface of the non-pigmented CBE and, in a patchy distribution, within CBE cells in all 8 enucleated globes. It also co-localised with the type II collagen of vitreous, where present, in these eyes. Opticin was present in 16 of the 19 PHMs/ERMs, where it was arranged in layers (10 membranes), diffusely (4 membranes) or in foci (2 membranes). Where in a layered pattern, opticin co-localised with vitreous type II collagen incorporated into the membrane, whereas the other two patterns did not co-localise with type II collagen labelling. We concluded that even in advanced proliferative retinal disease, the CBE continues to express and secrete opticin. Opticin was co-distributed with vitreous type II collagen and was also present in the pre-retinal membranes of proliferative retinopathies, where it could play a role in their development.
这项研究旨在确定外切蛋白（opticin）在病理性眼部组织中的分布，外切蛋白是由非色素睫状体上皮（CBE）分泌的细胞外基质小亮氨酸丰富重复蛋白。这些病理性眼部组织包括增生性糖尿病视网膜病变（PDR）、视网膜中央静脉阻塞（CRVO）和增生性玻璃体视网膜病变（PVR）患者的后玻璃体膜（PHM）和视网膜前膜（ERM）。通过免疫组织化学分析 8 例 PDR、CRVO 或 PVR 患者的眼球和 11 例手术切除的 PHM/ERM 中 opticin、玻璃体（由 II 型胶原抗体界定）和血管（使用 CD31 和 CD34 抗体作为内皮标志物）的存在和分布。外切蛋白存在于非色素性 CBE 的基底表面，并且在 8 例眼球中，CBE 细胞中呈斑片状分布。它还与这些眼中存在的玻璃体 II 型胶原共定位。在 19 例 PHM/ERM 中，有 16 例存在外切蛋白，其排列方式为层状（10 个膜）、弥漫状（4 个膜）或局灶状（2 个膜）。在层状模式下，外切蛋白与整合到膜中的玻璃体 II 型胶原共定位，而其他两种模式则不与 II 型胶原标记共定位。我们得出结论，即使在晚期增生性视网膜疾病中，CBE 仍继续表达和分泌外切蛋白。外切蛋白与玻璃体 II 型胶原共分布，也存在于增生性视网膜病变的视网膜前膜中，它可能在其发生发展中发挥作用。
