芒果果实提取物对氧化应激细胞毒性的保肝活性研究。
A search for hepatoprotective activity of fruit extract of Mangifera indica L. against oxidative stress cytotoxicity.
机构信息
Faculty of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Plant Foods Hum Nutr. 2010 Mar;65(1):83-9. doi: 10.1007/s11130-010-0161-9.
Mango (Mangifera indica L.) and their components are commonly used in folk medicine for many curative effects. The protective effects of different concentrations of aqueous extract of Mangifera indica L. fruit (Mango Extract) (20, 50 and 100 microg/ml) and also gallic acid (100 microM) as a pure compound in the extract were examined against oxidative stress toxicity induced by cumene hydroperoxide (CHP) in isolated rat hepatocytes. The extracts and gallic acid (100 microM) protected the hepatocyte against all oxidative stress markers including cell lysis, ROS generation, lipid peroxidation, glutathione depletion, mitochondrial membrane potential decrease, lysosomal membrane oxidative damage and cellular proteolysis. Mango Extracts (20, 50 and 100 microg/ml) were more effective than gallic acid (100 microM) in protecting hepatocytes against CHP induced lipid peroxidation. On the other hand gallic acid (100 microM) acted more effective than Mango Extracts (20, 50 and 100 microg/ml) at preventing lysosomal membrane damage. In addition H(2)O(2) scavenging effect of all extracts were determined in hepatocytes and compared with gallic acid (100 microM). There were no significance differences (P<0.05) between all plant extracts and gallic acid (100 microM) in H(2)O(2) scavenging activity. These results suggest a hepatoprotective role for Mango Extract against liver injury associated with oxidative stress.
芒果（Mangifera indica L.）及其成分在民间医学中常用于多种疗效。不同浓度的芒果（Mangifera indica L.）果实水提物（芒果提取物）（20、50 和 100 μg/ml）和纯化合物没食子酸（100 μM）对过氧化枯烯（CHP）诱导的大鼠肝细胞氧化应激毒性的保护作用进行了研究。提取物和没食子酸（100 μM）可保护肝细胞免受所有氧化应激标志物的影响，包括细胞溶解、ROS 生成、脂质过氧化、谷胱甘肽耗竭、线粒体膜电位降低、溶酶体膜氧化损伤和细胞蛋白水解。芒果提取物（20、50 和 100 μg/ml）在保护肝细胞免受 CHP 诱导的脂质过氧化方面比没食子酸（100 μM）更有效。另一方面，没食子酸（100 μM）在预防溶酶体膜损伤方面比芒果提取物（20、50 和 100 μg/ml）更有效。此外，还在肝细胞中测定了所有提取物的 H2O2 清除作用，并与没食子酸（100 μM）进行了比较。所有植物提取物与没食子酸（100 μM）在 H2O2 清除活性方面均无显着差异（P<0.05）。这些结果表明，芒果提取物对与氧化应激相关的肝损伤具有肝保护作用。
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