Department of Surgery and Integrated Clinic, UNESP-Univ Estadual Paulista, Brazil.
Bone. 2010 Apr;46(4):1021-9. doi: 10.1016/j.bone.2009.12.016. Epub 2009 Dec 28.
The influence of an estrogen-deficient state was evaluated in this study and also its treatments with estrogen (E(2)) or with raloxifene (RLX) on the expression of osteocalcin during the periods of the chronology of the alveolar bone healing process (7, 14, 21, 28 and 42 post-extraction days) by means of immunohistochemistry reactions and histomorphometric analysis. Rats (200-220 g) with oestrus cycles normal were either OVX or sham-operated and divided into four groups: sham, OVX control (OVX/O), estrogen (OVX/E(2); 17 beta-estradiol, 400 microg/mo) and raloxifene (OVX/RLX; 1 mg/kg bw/d) groups. Histomorphometric analysis showed the sham group presented the highest mean value of bone formation post-extraction. The reaction of immunohistochemistry for osteocalcin presented stronger expression of osteocalcin with predominance at 14 and 21 days on sham group. The OVX/RLX group presented better results than OVX/E(2), considering the expression of osteocalcin in osteoblastic lineage cells, but still inferior than the sham group. It was concluded that ovariectomy decreases the mineralization process and the osteocalcin expression during the chronology of the alveolar healing process that is not totally recovered with estrogen replacement or raloxifene treatment.
本研究评估了雌激素缺乏状态及其治疗方法对牙槽骨愈合过程各时期（拔牙后 7、14、21、28 和 42 天）骨钙素表达的影响，采用免疫组织化学反应和组织形态计量学分析。发情周期正常的大鼠（200-220 克）进行卵巢切除术（OVX）或假手术，并分为四组：假手术组、OVX 对照组（OVX/O）、雌激素组（OVX/E2；17β-雌二醇，400μg/月）和雷洛昔芬组（OVX/RLX；1mg/kg bw/d）。组织形态计量学分析显示，假手术组拔牙后骨形成的平均数值最高。骨钙素免疫组织化学反应显示，假手术组在 14 天和 21 天的骨钙素表达最强。与雌激素替代或雷洛昔芬治疗相比，OVX/RLX 组在成骨细胞谱系细胞中骨钙素的表达更好，但仍低于假手术组。结论：卵巢切除术可降低牙槽骨愈合过程中矿化过程和骨钙素的表达，而雌激素替代或雷洛昔芬治疗并不能完全恢复。
