Troian-Michel Carolina Horn, Só Bruna Barcelos, Schuch Lauren Frenzel, Stelter Grazielle Oliveira, Schmidt Tuany Rafaeli, Schorr Joana Letícia, Campagnol Daniela, Garcez Tuane Nerissa Alves, Só Gabriel Barcelos, Arrué Thalita Ayres, Weissheimer Theodoro, Martins Manoela Domingues, Só Marcus Vinicius Reis
Department of Endodontics, School of Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, Brazil.
Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, Brazil.
Int Endod J. 2025 Jul;58(7):1042-1059. doi: 10.1111/iej.14234. Epub 2025 Apr 4.
Osteoporosis is a disease that is related to a higher prevalence and greater volumes of apical periodontitis. The purpose of this study was to evaluate the effect of ovariectomy-induced osteoporosis and high- or low-level laser photobiomodulation (PBM) on the repair of apical periodontitis (AP) in adult female Wistar rats.
Sixty female Wistar rats (n = 120) were randomly allocated into four control (n = 6 teeth) and 8 experimental (n = 12 teeth) groups, according to procedure and time of evaluation: healthy control (GCS14/GCS28), osteoporotic control (GCO14/GCO28), sham (SHAM14/SHAM28), osteoporotic (OVX14/OVX28), low-level laser (OVX-LLL14/OVX-LLL28) and high-level laser (OVX-GEM14/OVX-GEM28). All groups were submitted to bilateral ovariectomy, except for the healthy control and sham. Eight weeks later, AP was induced bilaterally in mandibular first molars' mesial roots in experimental groups, and after 21 days, their endodontic treatment (RCT) was conducted. For 14 or 28 days, PBM was applied three times/week, as follows: sham application (SHAM and OVX groups); low-level laser (OVX/LLL groups); defocused high-power laser (OVX/GEM groups). After the respective experimental periods, the animals were euthanized. Tibias and hemi-mandibula were obtained and submitted to computed microtomography. Bone volume fraction (BV/TV), trabeculae number (Tb.N), spacing (Tb.Sp) and thickness (Tb.Th) were obtained for tibias, while RCT apical limit, periodontal ligament and AP volumes (APvol) were recorded for hemi-mandibula. Data were subjected to statistical analysis through generalized estimating equations (GEE), ANOVA and chi-square tests (5% significance level).
Ovariectomy tibias showed lower BV/TV and Tb.N (p < .0001) and higher Tb.Th (p = .020) and Tb.Sp (p < .0001) when compared to sham groups. For hemi-mandibula, lower APvol values were found in SHAM14 when compared to OVX/LLL14 and OVX/GEM14 groups (p < .05), which were not different from each other. Over the 28-day period, the OVX/LLL28 group showed higher AP volumes when compared to OVX28 and OVX/GEM28 (p < .05). Analysing variables effects on APvol, time, PBM and RCT apical limit showed no effect (p > .05), while ovariectomy showed a significant increase (p = .004).
Ovariectomy-induced osteoporosis in Wistar rats led to a reduction in AP repair, and PBM was unable to counteract this effect.
骨质疏松症是一种与根尖周炎更高的患病率和更大的病变范围相关的疾病。本研究的目的是评估去卵巢诱导的骨质疏松症以及高或低水平激光光生物调节(PBM)对成年雌性Wistar大鼠根尖周炎(AP)修复的影响。
60只雌性Wistar大鼠(n = 120)根据评估程序和时间随机分为4个对照组(n = 6颗牙齿)和8个实验组(n = 12颗牙齿):健康对照组(GCS14/GCS28)、骨质疏松症对照组(GCO14/GCO28)、假手术组(SHAM14/SHAM28)、骨质疏松症组(OVX14/OVX28)、低水平激光组(OVX-LLL14/OVX-LLL28)和高水平激光组(OVX-GEM14/OVX-GEM28)。除健康对照组和假手术组外,所有组均进行双侧卵巢切除术。8周后,在实验组大鼠下颌第一磨牙近中根双侧诱导AP,21天后进行根管治疗(RCT)。在14天或28天内,每周进行3次PBM,具体如下:假照射(SHAM组和OVX组);低水平激光(OVX/LLL组);散焦高功率激光(OVX/GEM组)。在各自的实验期结束后,对动物实施安乐死。获取胫骨和半侧下颌骨并进行计算机断层扫描。测量胫骨的骨体积分数(BV/TV)、骨小梁数量(Tb.N)、间距(Tb.Sp)和厚度(Tb.Th),同时记录半侧下颌骨的RCT根尖界限、牙周膜和AP体积(APvol)。通过广义估计方程(GEE)、方差分析和卡方检验(显著性水平为5%)对数据进行统计分析。
与假手术组相比,去卵巢大鼠的胫骨显示出较低的BV/TV和Tb.N(p < 0.0001)以及较高的Tb.Th(p = 0.020)和Tb.Sp(p < 0.0001)。对于半侧下颌骨,与OVX/LLL14和OVX/GEM14组相比,SHAM14组的APvol值较低(p < 0.05),而这两组之间无差异。在28天的观察期内,与OVX28和OVX/GEM28组相比,OVX/LLL28组的AP体积较高(p < 0.05)。分析各变量对APvol的影响,时间、PBM和RCT根尖界限均无影响(p > 0.05),而去卵巢则显示出显著增加(p = 0.004)。
Wistar大鼠去卵巢诱导的骨质疏松症导致AP修复减少,且PBM无法抵消这种影响。