Department of Surgery, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA.
J Surg Res. 2011 Jul;169(1):125-31. doi: 10.1016/j.jss.2009.09.021. Epub 2009 Oct 1.
The differences and efficacy of standard preservation solutions in kidney transplantation, University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK), remain a topic of debate in recent clinical studies. P-selectins represent glycoproteins expressed on endothelial cells and platelets responsible for the earliest events in ischemia/reperfusion injury in kidney transplantation. This study aimed to compare the levels of P-selectin expression between cold preserved kidney tissues in UW and HTK solutions. Thirty kidneys were procured from male Lewis rats and stored in cold (4°C) solutions for periods of 4, 12, 16, 20, and 24h. Group 1 (n=15) kidneys were stored in UW solutions, and group 2 (n=15) kidneys were submerged in HTK solutions. At the end of each time point, the kidneys underwent preparation and levels of P-selectin expression in the tissues were measured using Immunoblot analyses and adjusted volumetric quantification of Western blot signals. For all periods of cold preservation, P-selectin expression was significantly down-regulated in kidney tissues stored in UW compared with HTK solutions (P<0.001). In summary, UW demonstrated a significant benefit over HTK solution in down-regulating P-selectin expression in cold preserved kidney grafts.
在最近的临床研究中，威斯康星大学（UW）和组氨酸-色氨酸-酮戊二酸（HTK）等标准保存液在肾移植中的差异和疗效仍然存在争议。P-选择素是内皮细胞和血小板上表达的糖蛋白，负责肾移植中缺血/再灌注损伤的最早事件。本研究旨在比较 UW 和 HTK 溶液中冷保存肾组织中 P-选择素表达水平。从雄性 Lewis 大鼠中获取 30 个肾脏，并在 4°C 溶液中储存 4、12、16、20 和 24 小时。第 1 组（n=15）肾脏储存在 UW 溶液中，第 2 组（n=15）肾脏浸泡在 HTK 溶液中。在每个时间点结束时，对肾脏进行准备，并使用免疫印迹分析测量组织中 P-选择素表达水平，并对 Western blot 信号进行体积定量调整。在所有冷保存期，与 HTK 溶液相比，UW 中保存的肾组织中 P-选择素表达显著下调（P<0.001）。总之，UW 在下调冷保存肾移植物中 P-选择素表达方面优于 HTK 溶液。
