Department of Pediatrics, Division of Pediatric Neuroscience, Medical University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria.
Neurochem Int. 2010 Feb;56(3):522-7. doi: 10.1016/j.neuint.2009.12.013. Epub 2009 Dec 30.
Strain-dependence of performance in the Morris water maze (MWM) has been reported but information on underlying differences at the protein level as well as linkage of hippocampal proteins to memory is limited. No data are available on differences in hippocampal protein levels between (within-strain) "good" and "bad" performers in the MWM. Hippocampi of wild-caught mouse strain Apodemus sylvaticus (AS), laboratory inbred mouse strain C57BL/6J and wild-derived inbred mouse strain PWD/PhJ, that were tested in the MWM, were taken and postsynaptic density protein 95, neuronal marker protein NeuN, dendritic spine protein drebrin, synapsins 1a and 1b and synaptosomal protein syntaxin of the SNARE complex were determined by immunoblotting. These mouse strains with known different performance in the MWM showed different hippocampal protein patterns and by the use of yoked controls proteins could be identified as linked to memory formation. When mice, classified as good or bad performers by the median of time spent in the target quadrant on day 12 of the MWM, experiments, NeuN was discriminating good from bad performers in AS at the stringent statistical level of P<0.0001, an effect not seen in the corresponding yoked controls. Strain-dependence of performance in the MWM was reflected by different hippocampal protein levels. Most hippocampal proteins given above were linked to memory formation in the MWM. The finding that the neuronal protein NeuN is able to discriminate between good and bad performers in the MWM may be of major interest and may open a new area in the search for protein markers of spatial memory performance.
在 Morris 水迷宫 (MWM) 中已经报道了表现的应变依赖性，但关于蛋白质水平下的潜在差异以及海马蛋白与记忆的联系的信息有限。在 MWM 中，“好”和“差”表现者之间的海马蛋白水平差异尚无数据。通过免疫印迹法测定了在 MWM 中测试的野生捕获的 Apodemus sylvaticus (AS) 小鼠品系、实验室近交的 C57BL/6J 小鼠品系和野生衍生的近交 PWD/PhJ 小鼠品系的海马突触后密度蛋白 95、神经元标记蛋白 NeuN、树突棘蛋白 drebrin、突触素 1a 和 1b 和 SNARE 复合物的突触小体蛋白 syntaxin。这些在 MWM 中具有已知不同表现的小鼠品系显示出不同的海马蛋白模式，并通过使用配对对照物，可确定这些蛋白与记忆形成有关。当根据 MWM 第 12 天在目标象限花费的时间中位数将小鼠分类为表现良好或表现不佳的小鼠时，在严格的统计学水平 P<0.0001 下，AS 中的 NeuN 可将良好与不良表现者区分开来，而在相应的配对对照物中则没有观察到这种情况。MWM 中表现的应变依赖性反映了不同的海马蛋白水平。上述大多数海马蛋白都与 MWM 中的记忆形成有关。神经元蛋白 NeuN 能够区分 MWM 中表现良好和表现不佳的个体的发现可能具有重要意义，并可能为寻找空间记忆表现的蛋白质标志物开辟新领域。
