Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.
Amino Acids. 2012 Oct;43(4):1739-49. doi: 10.1007/s00726-012-1258-2. Epub 2012 Mar 9.
A series of individual proteins have been linked to performance in the Morris water maze (MWM) but no global effects have been reported. It was therefore the aim of the study to show which proteins were strain-independent, global factors for training in the MWM. Strains C57BL/6J, apodemus sylvaticus and PWD/PhJ were used. MWM and gels from trained animals were from a previous own study and corresponding yoked groups were generated. Hippocampal proteins were extracted and run on two-dimensional gel electrophoresis. Spots with different expressional levels between trained and yoked groups were punched and identified by mass spectrometry (nano-LC-ESI-MS/MS, ion trap). Two-way ANOVA with two factors (strain and training) was carried out and a Bonferroni test was used to compare groups. 12 proteins from several pathways and cascades showed different levels in trained mice versus corresponding yoked animals in all strains tested. Four out of these proteins were verified by immunoblotting: beta-synuclein, profilin 2, nucleoside diphosphate kinase A (NME1) and isocitrate dehydrogenase 3. Four proteins verified by immunoblotting could be shown to be involved in training in the MWM as a global effect, independent of the strain tested.
一系列的个体蛋白已经与 Morris 水迷宫（MWM）中的表现相关联，但没有报道全局效应。因此，本研究旨在表明哪些蛋白是 MWM 训练中的独立于品系的全局因素。使用了 C57BL/6J、黑线姬鼠和 PWD/PhJ 品系。MWM 和来自训练动物的凝胶来自之前的自有研究，并生成了相应的配对组。提取海马蛋白并在二维凝胶电泳上运行。用质谱法（纳升 LC-ESI-MS/MS，离子阱）对训练组和配对组之间表达水平不同的斑点进行打孔和鉴定。用具有两个因素（品系和训练）的双向方差分析和 Bonferroni 检验来比较组。在所有测试的品系中，来自几个途径和级联的 12 种蛋白在训练小鼠与相应的配对动物中表现出不同的水平。其中 4 种蛋白通过免疫印迹得到验证：β-突触核蛋白、原肌球蛋白 2、核苷二磷酸激酶 A（NME1）和异柠檬酸脱氢酶 3。通过免疫印迹验证的 4 种蛋白可以被证明是作为全局效应参与 MWM 训练的，与测试的品系无关。
