Xu Fenghua, Liu Guiyang, Zhang Qiang, Siegel Ronald A
School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Front Biosci (Elite Ed). 2010 Jan 1;2(2):657-67. doi: 10.2741/e121.
Two phenylboronic acid based glucose-sensitive hydrogels, A.PBA-DMAPMA-EGDMA and A.PBA-PEG, were initially prepared by free-radical polymerization. Swelling properties of the gels were studied by determining the diameter changes in different buffer solutions, with or without glucose or fructose. The hydrogels were designed as "valves" to control the flow of glucose solutions. The results showed that gel A.PBA-DMAPMA-EGDMA was sensitive to pH and glucose, but not to fructose. It shrunk in weak basic solution and the addition of glucose made it shrink more. In this gel PBA moiety and glucose is supposed to form a 1:2 bis-bidentate complex. Hydrogel A.PBA-PEG was sensitive to pH, glucose and fructose, all of which made it swell in weak basic solution. A 1:1 complex is believed to form between PBA and glucose/fructose in this gel. All the stimuli-responses are reversible and the glucose-responses occurred in the range of the physiological/pathological glucose level. Both A.PBA-DMAPMA-EGDMA and A.PBA-PEG exhibited sufficient volume change to the alteration of glucose concentration and could be employed as a "valve" to control liquid flow in weak basic solution.
最初通过自由基聚合制备了两种基于苯硼酸的葡萄糖敏感水凝胶，即A.PBA-DMAPMA-EGDMA和A.PBA-PEG。通过测定在不同缓冲溶液（含或不含葡萄糖或果糖）中的直径变化来研究凝胶的溶胀性能。这些水凝胶被设计成“阀门”以控制葡萄糖溶液的流动。结果表明，凝胶A.PBA-DMAPMA-EGDMA对pH和葡萄糖敏感，但对果糖不敏感。它在弱碱性溶液中收缩，加入葡萄糖会使其收缩得更厉害。在这种凝胶中，PBA部分和葡萄糖应该形成1:2的双齿络合物。水凝胶A.PBA-PEG对pH、葡萄糖和果糖敏感，所有这些都会使其在弱碱性溶液中溶胀。据信在这种凝胶中PBA与葡萄糖/果糖形成1:1的络合物。所有的刺激响应都是可逆的，并且葡萄糖响应发生在生理/病理葡萄糖水平范围内。A.PBA-DMAPMA-EGDMA和A.PBA-PEG对葡萄糖浓度的变化都表现出足够的体积变化，并且可以用作“阀门”来控制弱碱性溶液中的液体流动。
