Divisions of Infectious Diseases, Los Angeles Biomedical Research Institute, Harbor-University of California at Los Angeles (UCLA) Medical Center, USA.
J Infect Dis. 2010 Feb 1;201(3):473-7. doi: 10.1086/649901.
We determined reactivity of human blood to a vaccine based on the recombinant N-terminus of candidal Als3p (rAls3p-N) in preparation for future clinical trials. Healthy donor plasma had high immunoglobulin G titers (median, 1:51,200) and lower immunoglobulin A (median, 1:3,200) and immunoglobulin E (median, 1:128) titers to rAls3p-N by enzyme-linked immunosorbent assay. rAls3p-N stimulated interferon gamma (IFN-gamma) and interleukin (IL)-17, but not IL-4, from donor lymphocytes by enzyme-linked immunosorbent spot assay and IL-12 p70, IFN-gamma, IL-17, and IL-10 by cytometric bead array. Donors reacted to diverse immunodominant epitopes. Thus, facile humoral and cellular assays can monitor immune responses to the rAls3p-N vaccine in planned clinical trials.
我们测定了人类血液对基于念珠菌 Als3p 重组 N 端（rAls3p-N）的疫苗的反应性，为未来的临床试验做准备。通过酶联免疫吸附试验，健康供体血浆对 rAls3p-N 的免疫球蛋白 G 滴度（中位数为 1:51,200）较高，免疫球蛋白 A（中位数为 1:3,200）和免疫球蛋白 E（中位数为 1:128）滴度较低。酶联免疫斑点分析显示，rAls3p-N 可刺激供体淋巴细胞产生干扰素 γ（IFN-γ）和白细胞介素 17（IL-17），但不刺激白细胞介素 4（IL-4）。通过流式细胞术检测到细胞因子珠阵列产生白细胞介素 12 p70、干扰素 γ、白细胞介素 17 和白细胞介素 10。供体对多种免疫优势表位有反应。因此，简单的体液和细胞检测可以监测计划临床试验中 rAls3p-N 疫苗的免疫反应。
