Institute of Medical Engineering, National Taiwan University, Taipei, Taiwan 100, Republic of China.
J Biomater Sci Polym Ed. 2010;21(1):17-36. doi: 10.1163/156856209X410139.
Polyhydroxyalkanoates (PHAs) are a newer family of biomaterials for tissue-engineering applications. The objective of this study is to investigate the behavior of human mesenchymal stem cells (hMSCs) grown on various PHA films. The surface characteristics of PHA co-polymer films were varied by the content of 3-hydroxyvalerate (HV) or 3-hydroxyhexanoate (HHx) and by the film preparation methods such as compression-molding and solvent-casting. Hyaluronic acid (HA) was further applied to modify the surface properties of PHA membranes. As HV content increased, the crystallinity and the hydrophobicity of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) membranes decreased and the metabolic activity of hMSCs raised, although the distribution and morphology of hMSCs did not show significant variation. Hyaluronic acid (HA) coating on PHA membranes could improve the metabolic activity and reduce the death rate of hMSCs. Aggregates and spheroidal clusters of hMSCs were found on the surface of cast poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) membranes. The growth of hMSCs was remarkably influenced by various surface characteristics of the PHA films.
聚羟基烷酸酯（PHAs）是一类新型的生物材料，可用于组织工程应用。本研究旨在研究在不同 PHA 薄膜上生长的人骨髓间充质干细胞（hMSCs）的行为。通过改变 3-羟基戊酸（HV）或 3-羟基己酸（HHx）的含量以及压缩成型和溶剂浇铸等薄膜制备方法来改变共聚酯 PHB 薄膜的表面特性。进一步将透明质酸（HA）应用于修饰 PHA 膜的表面性能。随着 HV 含量的增加，聚（3-羟基丁酸-co-3-羟基戊酸）（PHBV）膜的结晶度和疏水性降低，hMSCs 的代谢活性提高，尽管 hMSCs 的分布和形态没有显示出明显的变化。透明质酸（HA）涂层可提高 PHA 膜的代谢活性并降低 hMSCs 的死亡率。在浇铸的聚（3-羟基丁酸-co-3-羟基己酸）（PHBHHx）膜表面发现 hMSCs 的聚集和球形簇。hMSCs 的生长受到 PHA 薄膜各种表面特性的显著影响。
