Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan, Taiwan 320, Republic of China.
J Biomater Sci Polym Ed. 2012;23(1-4):1-26. doi: 10.1163/092050610X541386. Epub 2011 Jul 12.
The influence of the topographic morphology of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) films on human mesenchymal stem cells (hMSCs) was investigated in this study. PHBHHx films with various surface characteristics were prepared by compression-molding, solvent-casting and electrospinning. The adhesion, proliferation and differentiation behaviors of hMSCs were significantly modulated by the surface characteristics of these films. HMSCs could aggregate and form cellular clusters on the cast PHBHHx films, and the time to form cellular aggregates increased as the surface roughness increased. The aggregated hMSCs on the cast films kept their original surface markers and presented much higher viability during the regular culture and lower differentiation ability upon osteogenic induction than the spread cells on the compression-molded films and TCPS. HMSCs spread well and showed a specific orientation on the surface of the random electrospun fibrous films, they were not able to migrate into the interior of electrospun fibrous films, and they revealed the highest viability during the regular culture but a lower differentiation activity upon osteogenic induction. The electrospun fibrous PHBHHx films could serve as a suitable substrate for large quantity culturing of hMSCs when undifferentiated hMSCs are desired.
本研究考察了聚（3-羟基丁酸-co-3-羟基己酸）（PHBHHx）薄膜的地形形貌对人骨髓间充质干细胞（hMSCs）的影响。通过压缩成型、溶剂浇铸和静电纺丝制备了具有不同表面特性的 PHBHHx 薄膜。hMSCs 的粘附、增殖和分化行为显著受到这些薄膜表面特性的调节。hMSCs 可以在浇铸的 PHBHHx 薄膜上聚集并形成细胞团，并且随着表面粗糙度的增加，形成细胞团的时间增加。聚集在浇铸薄膜上的 hMSCs 保持其原始的表面标志物，并且在常规培养中具有更高的活力，在成骨诱导时分化能力更低，而在压缩成型薄膜和 TCPS 上的扩散细胞则相反。hMSCs 在随机静电纺丝纤维薄膜的表面上很好地扩散并呈现出特定的取向，它们不能迁移到静电纺丝纤维薄膜的内部，并且在常规培养中具有最高的活力，但在成骨诱导时分化活性较低。当需要未分化的 hMSCs 时，静电纺丝纤维 PHBHHx 薄膜可用作大量培养 hMSCs 的合适基质。
