Hu Ya-Jun, Wei Xing, Zhao Wei, Liu Yuan-Sheng, Chen Guo-Qiang
Multidisciplinary Research Center, Shantou University, No. 243, Daxue Road, Shantou, 515063 Guangdong, China.
Acta Biomater. 2009 May;5(4):1115-25. doi: 10.1016/j.actbio.2008.09.021. Epub 2008 Oct 17.
As a new member of the polyhydroxyalkanoate (PHA) family, poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) (PHBVHHx) was produced by recombinant Aeromonas hydrophila 4AK4. PHBVHHx showed a rougher surface and had higher hydrophobicity than the well-studied polymers poly(L-lactic acid) (PLA) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Human bone marrow mesenchymal stem cells (MSCs) adhered better on PHBVHHx film than on tissue culture plates (TCPs), PLA film and PHBHHx film. The cell number on the PHBVHHx film was 115% higher than that on the TCPs, 66% higher than on the PHBHHx film and 263% higher than on the PLA film (p<0.01). PHBVHHx also supported the osteogenic differentiation of MSCs. Previous studies have shown that all PHA polymers tested were either poorer than or equal to TCPs for supporting cell growth. PHBVHHx is the only PHA polymer to significantly increase cell numbers compared with TCPs. These data demonstrate that PHBVHHx could be a promising biomaterial for bone tissue engineering.
作为聚羟基脂肪酸酯（PHA）家族的新成员，聚（3-羟基丁酸酯-co-3-羟基戊酸酯-co-3-羟基己酸酯）（PHBVHHx）由重组嗜水气单胞菌4AK4产生。与经过充分研究的聚合物聚（L-乳酸）（PLA）和聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）相比，PHBVHHx的表面更粗糙且疏水性更高。人骨髓间充质干细胞（MSCs）在PHBVHHx膜上的黏附比在组织培养板（TCPs）、PLA膜和PHBHHx膜上更好。PHBVHHx膜上的细胞数量比TCPs上的高115%，比PHBHHx膜上的高66%，比PLA膜上的高263%（p<0.01）。PHBVHHx还支持MSCs的成骨分化。先前的研究表明，所有测试的PHA聚合物在支持细胞生长方面要么比TCPs差，要么与TCPs相当。与TCPs相比，PHBVHHx是唯一能显著增加细胞数量的PHA聚合物。这些数据表明，PHBVHHx可能是一种有前途的骨组织工程生物材料。
