[选择球孢白僵菌（子囊菌门：麦角菌科）分离株用于控制微小牛蜱（蜱螨亚纲：硬蜱科）]

[Selection of isolates of entomopathogenic fungus Beauveria bassiana (Ascomycetes: Clavicipitaceae) for control of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)].

作者信息

Barci Leila A G, de Almeida José Eduardo M, de Campos Nogueira Adriana H, Zappelini Luciano O, do Prado Angelo P

机构信息

Centro de Pesquisa e Desenvolvimento de Sanidade Animal-CPDSA, Instituto Biológico, Av. Conselheiro Rodrigues Alves 1.252, CEP 04.014-002, São Paulo-SP, Brazil.

出版信息

Rev Bras Parasitol Vet. 2009 Dec;18 Suppl 1:7-13. doi: 10.4322/rbpv.018e1002.

DOI:10.4322/rbpv.018e1002

PMID:20040184

Abstract

This study was carried out to select isolates of the entomopathogenic fungus Beauveria bassiana with pathogenic potential to control the Rhipicephalus (Boophilus) microplus tick. The effectiveness of thirty isolates was first tested at a concentration of 5 x 108 conidia.mL(-1). Of these, eight were evaluated (IBCB01, IBCB02, IBCB07, IBCB17, IBCB21, IBCB74, IBCB149, IBCB165) and showed an effectiveness between 90 and 99%; thirteen (IBCB03, IBCB14, IBCB16, IBCB24, IBCB95, IBCB97, IBCB102, IBCB141, IBCB146, IBCB147, IBCB150, IBCB154, IBCB157) between 80 and 89,5%; six (IBCB47, IBCB75, IBCB84, IBCB145, IBCB161, IBCB164) between 70 and 79%, and only two (IBCB13 and IBCB143) had lower pathogenicity (70% or below). In the second step of the study, the five more effective strains in the first phase of the experiment (IBCB01, IBCB07, IBCB21, IBCB66, IBCB165) were analyzed comparatively. Based on in vitro results, it can be concluded that IBCB66 and IBCB21 are the isolates with higher potential for field control of R. (B.) microplus. IBCB01, IBCB07, IBCB21, IBCB66 e IBCB165 isolates were submitted to a conidial production test using a rice-based substrate. The best mass production of the entomopathogenic fungus was obtained with the IBCB66 strain.

摘要

本研究旨在筛选对微小牛蜱具有致病潜力的球孢白僵菌昆虫病原真菌分离株。首先在浓度为5×10⁸ 分生孢子·mL⁻¹ 的条件下测试了30个分离株的有效性。其中，8个（IBCB01、IBCB02、IBCB07、IBCB17、IBCB21、IBCB74、IBCB149、IBCB165）表现出90%至99%的有效性；13个（IBCB03、IBCB14、IBCB16、IBCB24、IBCB95、IBCB97、IBCB102、IBCB141、IBCB146、IBCB147、IBCB150、IBCB154、IBCB157）在80%至89.5%之间；6个（IBCB47、IBCB75、IBCB84、IBCB145、IBCB161、IBCB164）在70%至79%之间，只有2个（IBCB13和IBCB143）致病性较低（70%或以下）。在研究的第二步，对实验第一阶段中五个效果最佳的菌株（IBCB01、IBCB07、IBCB21、IBCB66、IBCB165）进行了比较分析。根据体外实验结果，可以得出结论，IBCB66和IBCB21是对微小牛蜱进行田间防治潜力较高的分离株。IBCB01、IBCB07、IBCB21、IBCB66和IBCB165分离株使用基于大米的基质进行了分生孢子生产测试。昆虫病原真菌的最佳大量生产是用IBCB66菌株获得的。