Sun Ming, Ren Qiaoyun, Guan Guiquan, Li Yufeng, Han Xueqing, Ma Chao, Yin Hong, Luo Jianxun
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, PR China.
Parasitol Int. 2013 Oct;62(5):412-5. doi: 10.1016/j.parint.2013.04.008. Epub 2013 May 4.
Owing to the need to combat the spread of acaricide-resistant ticks, the development of long-term biological control has become a hot topic for tick control. In this study, we investigated the pathogenicity of three Beauveria bassiana isolates on the engorged female Rhipicephalus (Boophilus) microplus ticks using different conidial concentrations. The results showed that B. bassiana B.bAT17 was highly pathogenic against engorged R. (B.) microplus females, resulting in lethal time (LT50 and LT90) of 7.14 and 9.33 days at a concentration of 10(9)conidia/ml. R. (B.) microplus females treated with B. bassiana B.bAT17 significantly reduced the amount of ovipositioning; and most ticks died before they could begin to oviposit. Proteases and chitinases were analyzed in order to establish a screening method for identification of high virulent strains. This study has confirmed the significant pathogenic effect of entomopathogenic fungi against engorged R. (B.) microplus females in China, and further studies on the efficiency of the fungus against ticks in the field are required.
由于需要应对抗杀螨剂蜱虫的传播，长期生物防治的发展已成为蜱虫防治的热门话题。在本研究中，我们使用不同的分生孢子浓度，研究了三种球孢白僵菌分离株对饱血雌性微小牛蜱的致病性。结果表明，球孢白僵菌B.bAT17对饱血微小牛蜱雌性具有高致病性，在浓度为10(9)个分生孢子/毫升时，致死时间（LT50和LT90）分别为7.14天和9.33天。用球孢白僵菌B.bAT17处理的微小牛蜱雌性产卵量显著减少；大多数蜱在开始产卵前死亡。分析了蛋白酶和几丁质酶，以建立一种筛选高毒力菌株的方法。本研究证实了昆虫病原真菌对中国饱血微小牛蜱雌性具有显著的致病作用，还需要进一步研究该真菌在田间对蜱虫的防治效果。
