[测定球孢白僵菌IBCB66菌株（子囊菌门：麦角菌科）对微小牛蜱（蜱螨亚纲：硬蜱科）的LC90和LT90以用于防治]
[Determination of LC 90 and LT 90 of IBCB66 Beauveria bassiana (Ascomycetes: Clavicipitaceae) isolate for Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) control].
作者信息
Barci Leila A G, de Almeida José Eduardo M, de Campos Nogueira Adriana H, do Prado Angelo P
机构信息
Centro de Pesquisa e Desenvolvimento de Sanidade Animal-CPDSA, Instituto Biológico, Av. Conselheiro Rodrigues Alves 1.252, CEP 04.014-002, São Paulo-SP, Brazil.
出版信息
Rev Bras Parasitol Vet. 2009 Dec;18 Suppl 1:34-9. doi: 10.4322/rbpv.018e1006.
The objective of this research was to evaluate the pathogenicity and the virulence of the IBCB66 isolate of Beauveria bassiana on infected larvae of Rhipicephalus (Boophilus) microplus. The IBCB66 fungus strain was used as standard isolates of B. bassiana against R. (B.) microplus larvae. The larval bioassay tests using the IBCB66 isolate were carried out to determine the (Lethal Concentration) LC50, LC90, (Lethal Time) LT50 and LT90. The IBCB66 fungus strain was tested at six different concentrations (5x10(6), 10(7), 5x10(7), 10(8), 5x10(8) and 10(9) conidia.mL(-1)) to determine the percentage of larval mortality. In addition, a Probit analysis was also performed. Total larval mortality was observed eighteen days after the beginning of the test in the group treated with 5x10(9) conidia.mL(1). The LC50 and LC90 were 3x10(7) and 5x10(8) respectively and the LT50 and LT90 were 10 and 16 days.
本研究的目的是评估球孢白僵菌IBCB66分离株对微小牛蜱感染幼虫的致病性和毒力。IBCB66真菌菌株被用作球孢白僵菌针对微小牛蜱幼虫的标准分离株。使用IBCB66分离株进行幼虫生物测定试验，以确定致死浓度（LC50、LC90）、致死时间（LT50和LT90）。IBCB66真菌菌株在六个不同浓度（5×10⁶、10⁷、5×10⁷、10⁸、5×10⁸和10⁹分生孢子·mL⁻¹）下进行测试，以确定幼虫死亡率百分比。此外，还进行了概率分析。在以5×10⁹分生孢子·mL⁻¹处理的组中，试验开始18天后观察到幼虫全部死亡。LC50和LC90分别为3×10⁷和5×10⁸，LT50和LT90分别为10天和16天。
Zotero 插件