Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical Schoo, Houston, TX 77030, USA.
Ther Drug Monit. 2010 Feb;32(1):112-5. doi: 10.1097/FTD.0b013e3181c6b0dc.
Because of a published report indicating significant interference of hydroxyzine with the particle-enhanced turbidimetric inhibition immunoassay (PENTINA) carbamazepine assay, we investigated whether such interference can be avoided by using the ADVIA Centaur carbamazepine assay. Both the Dimension Vista analyzer and ADVIA Centaur analyzer are available from Siemens Diagnostics. Aliquots of a drug-free serum pool were supplemented with various concentrations of hydroxyzine or cetirizine (0.05 microg/mL to 20 microg/mL covering therapeutic and toxic levels in serum) followed by analysis using both assays. We observed significant apparent carbamazepine concentrations using the PENTINA assay but no apparent carbamazepine level using the ADVIA Centaur assay. Because crossreactivity should be studied in the presence of the primary analyte, we also prepared a serum carbamazepine pool from patients receiving carbamazepine and then supplemented aliquots of this pool with various amounts of hydroxyzine or cetirizine followed by reanalyzing carbamazepine concentration using both assays. We observed falsely elevated carbamazepine values using the PENTINA assay but no interference was observed using the ADVIA Centaur assay. However, the falsely elevated carbamazepine values using the PENTINA assay were clinically significant at hydroxyzine or cetirizine concentrations expected in patients with severe overdoses with these drugs. We conclude that the ADVIA Centaur carbamazepine assay is free from interference of both hydroxyzine and cetirizine.
由于一份已发表的报告表明羟嗪会对用于检测卡马西平的粒子增强比浊抑制免疫测定法（PENTINA）产生显著干扰，我们研究了使用 ADVIA Centaur 卡马西平测定法是否可以避免这种干扰。Dimension Vista 分析仪和 ADVIA Centaur 分析仪均来自西门子诊断公司。将无药物血清池的等分试样用不同浓度的羟嗪或西替利嗪（0.05 微克/毫升至 20 微克/毫升，涵盖血清中的治疗和毒性水平）进行补充，然后用两种检测方法进行分析。我们观察到 PENTINA 检测法出现明显的卡马西平浓度，但 ADVIA Centaur 检测法没有明显的卡马西平水平。因为应该在存在主要分析物的情况下研究交叉反应性，我们还从接受卡马西平治疗的患者中制备了血清卡马西平池，然后用不同量的羟嗪或西替利嗪补充该池的等分试样，然后用两种检测方法重新分析卡马西平浓度。我们观察到 PENTINA 检测法出现假性升高的卡马西平值，但 ADVIA Centaur 检测法未观察到干扰。然而，在这些药物严重过量的患者中预期的羟嗪或西替利嗪浓度下，PENTINA 检测法出现假性升高的卡马西平值具有临床意义。我们得出结论，ADVIA Centaur 卡马西平测定法不受羟嗪和西替利嗪的干扰。
