Laboratoire de Bio-Ressources Naturelles Locales, Université Hassiba Ben Bouali de Chlef, 02000 Chlef, Algeria.
J Environ Public Health. 2009;2009:678495. doi: 10.1155/2009/678495. Epub 2009 Aug 12.
In the present study, the antibacterial effect of 20 lactic acid bacteria isolates from a traditional cheese was investigated. 6 isolates showed antibacterial effect against Gram positive bacteria. Streptococcus thermophilus T2 strain showed the wide inhibitory spectrum against the Gram positive bacteria. Growth and bacteriocin production profiles showed that the maximal bacteriocin production, by S. thermophilus T2 cells, was measured by the end of the late-log phase (90 AU ml(-1)) with a bacteriocine production rate of 9.3 (AU ml(-1)) h(-1). In addition, our findings showed that the bacteriocin, produced by S. thermophilus T2, was stable over a wide pH range (4-8); this indicates that such bacteriocin may be useful in acidic as well as nonacidic food. This preliminarily work shows the potential application of autochthonous lactic acid bacteria to improve safety of traditional fermented food.
在本研究中,我们研究了 20 株来自传统奶酪的乳酸菌分离株的抗菌作用。其中 6 株分离株对革兰氏阳性菌表现出抗菌作用。嗜热链球菌 T2 菌株对革兰氏阳性菌表现出广泛的抑制谱。生长和细菌素产生曲线表明,嗜热链球菌 T2 细胞的最大细菌素产量在对数末期(90 AU ml(-1)) 测量,细菌素产生率为 9.3 (AU ml(-1)) h(-1)。此外,我们的研究结果表明,由嗜热链球菌 T2 产生的细菌素在较宽的 pH 范围内(4-8)稳定;这表明这种细菌素可能在酸性和非酸性食品中都有用。这项初步工作表明,土著乳酸菌有可能应用于改善传统发酵食品的安全性。