Ravera Silvia, Bianco Bruno, Cugnoli Carlo, Panfoli Isabella, Calzia Daniela, Morelli Alessandro, Pepe Isidoro M
Department of Biology, University of Genoa, Genoa, Italy.
Bioelectromagnetics. 2010 May;31(4):270-6. doi: 10.1002/bem.20563.
The effects of extremely low frequency magnetic fields (ELF-MF) on acetylcholinesterase (AChE) activity of synaptosomal membranes were investigated. Sinusoidal fields with 50 Hz frequency and different amplitudes caused AChE activity to decrease about 27% with a threshold of about 0.74 mT. The decrease in enzymatic activity was independent of the time of permanence in the field and was completely reversible. Identical results were obtained with exposure to static MF of the same amplitudes. Moreover, the inhibitory effects on enzymatic activity are spread over frequency windows with different maximal values at 60, 200, 350, and 475 Hz. When synaptosomal membranes were solubilized with Triton, ELF-MF did not affect AChE activity, suggesting the crucial role of the membrane, as well as the lipid linkage of the enzyme, in determining the conditions for inactivation. The results are discussed in order to give an interpretation at molecular level of the macroscopic effects produced by ELF-MF on biological systems, in particular the alterations of embryo development in many organisms due to acetylcholine accumulation.
研究了极低频磁场（ELF-MF）对突触体膜乙酰胆碱酯酶（AChE）活性的影响。频率为50Hz、幅度不同的正弦磁场使AChE活性降低约27%，阈值约为0.74mT。酶活性的降低与在磁场中的持续时间无关，且完全可逆。暴露于相同幅度的静磁场也得到了相同的结果。此外，对酶活性的抑制作用分布在不同频率窗口，在60、200、350和475Hz时具有不同的最大值。当用曲拉通使突触体膜溶解时，ELF-MF不影响AChE活性，这表明膜以及酶的脂质连接在确定失活条件中起关键作用。对结果进行了讨论，以便从分子水平解释ELF-MF对生物系统产生的宏观效应，特别是许多生物体中由于乙酰胆碱积累导致的胚胎发育改变。
