Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University, Busan, Republic of Korea.
J Med Food. 2009 Dec;12(6):1206-12. doi: 10.1089/jmf.2009.0034.
We examined the beneficial effects of an active principle in kimchi, 3-(4'-hydroxyl-3',5'-dimethoxyphenyl)propionic acid (HDMPPA), on atherogenesis in apolipoprotein E knockout (apoE KO) mice. ApoE KO mice were fed an atherogenic diet containing 1% cholesterol (control group) with an intraperitoneal injection of chemically synthesized HDMPPA (10 mg/kg/day) (HDMPPA group) for 8 weeks. The aortic sinus atherosclerotic lesion size in the HDMPPA group (n = 10) was significantly smaller (control vs. HDMPPA, 280,790 vs. 165,409 microm(2), P < .001). The level of reactive oxygen species (ROS) in the HDMPPA group was lower by 14%, compared with the control group (P < .05). Aortic NADPH oxidase activity was significantly lower in the HDMPPA group than in the control group. HDMPPA suppressed the mRNA expression of p47phox and rac-1 of NADPH oxidase by 27.2% and 46.0%, respectively, compared with values of the control group. In conclusion, HDMPPA in kimchi may attenuate atherosclerosis in apoE KO mice through retardation of ROS generation via down-regulating the mRNA expression of p47phox and rac-1, which are the components of NADPH oxidase.
我们研究了泡菜中一种活性成分 3-(4'-羟基-3',5'-二甲氧基苯基)丙酸（HDMPPA）对载脂蛋白 E 基因敲除（apoE KO）小鼠动脉粥样硬化形成的有益作用。apoE KO 小鼠喂食含 1%胆固醇的动脉粥样硬化饮食（对照组），同时腹膜内注射化学合成的 HDMPPA（10 mg/kg/天）（HDMPPA 组）8 周。HDMPPA 组（n=10）主动脉窦粥样硬化病变面积明显较小（对照组 vs. HDMPPA，280,790 对 165,409 微米 2，P<.001）。与对照组相比，HDMPPA 组的活性氧（ROS）水平降低了 14%（P<.05）。HDMPPA 组主动脉 NADPH 氧化酶活性明显低于对照组。与对照组相比，HDMPPA 组 NADPH 氧化酶的 p47phox 和 rac-1 的 mRNA 表达分别降低了 27.2%和 46.0%。综上所述，泡菜中的 HDMPPA 可能通过下调 NADPH 氧化酶的 p47phox 和 rac-1 的 mRNA 表达来抑制 ROS 的生成，从而减轻 apoE KO 小鼠的动脉粥样硬化。
